LCMT1 (4A4) Mouse mAb

• 产品编号：CST-5691S      品牌：CST       原厂货号：5691S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

LCMT1 (4A4) Mouse mAb鼠单抗能够检测内源性LCMT1总蛋白水平。通过重组特异性小鼠LCMT1蛋白去免疫动物从而制备出此单克隆抗体。Leucine carboxyl methyltransferase-1 (LCMT1)是一个重要的哺乳动物甲基转移酶，它能酶促protein phosphatase 2A (PP2A)蛋白Leu309位点的催化C亚单位(1,2)。X-ray晶体学分析揭示人源 LCMT1和辅助因子S-adenosylmethionine complex的结构完整性能是PP2A适当的相互作用(3)。研究证明功能性 LCMT1对于PP2A去与B/B55和Bα调节亚单位家族一起形成异源三聚体的能力是关键的 (4,5)。减弱的LCMT1活性显示干扰有丝分裂和细胞存活(5)。在神经退行性模型中，增加的LCMT1的内源性水平、甲基化的PP2A (C)和Bα诱导发育和神经母细胞瘤细胞的分化(5-7)。

原厂资料：

Specificity / Sensitivity

LCMT1 (4A4) Mouse mAb recognizes endogenous levels of total LCMT1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to mouse LCMT1 protein.

Background

Leucine carboxyl methyltransferase-1 (LCMT1) is an important mammalian methyltransferase that methylates the catalytic C subunit of protein phosphatase 2A (PP2A) at Leu309 (1,2). X-ray crystallography analysis reveals that the structural integrity of human LCMT1 and cofactor S-adenosylmethionine complex enables proper interaction with PP2A (3). Evidence suggests that functional LCMT1 is crucial for the ability of PP2A to form heterotrimers with the B/B55 and Bα family of regulatory subunits (4,5). Impaired LCMT1 activity is shown to disrupt mitosis and cell survival (5). In neurodegenerative models, increases in endogenous levels of LCMT1, methylated PP2A (C), and Bα induce the development and differentiation of neuroblastoma cells (5-7).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.