IWS1 Antibody

产品编号：CST-5681S 品牌：CST 原厂货号：5681S
产品分类：抗体 > 一抗 > 蛋白特异性一抗
应用分类：

包装：

运保温度:

–20°C.

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描述：

IWS1 Antibody检测内源性IWS1总蛋白水平。通过人工合成人源IWS1蛋白氨基末端相应序列的多肽片段去免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。RNA聚合酶II一个大亚基的C末端结构域 (CTD)在这些不同步骤的整合中起到了重要的作用 (1,2)。IWS1蛋白与一个CTD结合转录延伸因子及H3伴侣的Spt6蛋白相互作用(1,2)。IWS1也招募另外一个CTD结合蛋白HYPB/Setd2组蛋白甲基转移酶到RNA聚合酶II复合物上，以使延伸偶联的H3K36三甲基化 (2)。因此，IWS1在一个大的复合物中与Spt6和HYPB/Setd2相互联系，并且在共转录水平上调节mRNA合成和在组蛋白甲基化(2)。

原厂资料：

Specificity / Sensitivity

IWS1 Antibody detects endogenous levels of total IWS1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human IWS1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Various steps in gene expression, such as mRNA processing, surveillance, export, and synthesis are coupled to transcription elongation (1,2). The C-terminal domain (CTD) of the large subunit of RNA polymerase II plays an important role in the integration of these different steps (1,2). IWS1 interacts with Spt6, a CTD-binding transcription elongation factor and H3 chaperone (1,2). IWS1 also recruits another CTD-binding protein, HYPB/Setd2 histone methyltransferase, to the RNA polymerase II complex for elongation-coupled H3K36 trimethylation (2). Thus, IWS1 links Spt6 and HYPB/Setd2 in a large complex and regulates mRNA synthesis and histone methylation at the co-transcriptional level (2).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody