NDRG2 Antibody

• 产品编号：CST-5667S      品牌：CST       原厂货号：5667S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NDRG2抗体识别内源性的NDRG2蛋白.多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源NDRG2蛋白的Lys25氨基酸残基序列一致。NDRG（N-Myc下游调控基因）家族，由NDRG1, NDRG2, NDRG3, and NDRG4组成，是一组与结构调节相关的蛋白，在细胞增殖、分化、凋亡和应激反应、细胞迁移/转移中起作用[1-3]。NDRG1最初在N-Myc基因敲除小鼠中被鉴定为一种上调的蛋白[1]。NDRG家族蛋白，特别是NDRG1和NDRG2被报道在各种癌症组织中下调表达，提示其功能角色为肿瘤抑制因子[4,5].NDRG1和 NDRG2由p53和 HIF-1上调表达，并通过相关的信号通路调控细胞凋亡[6-11]。NDRG1 和NDRG2 也和神经系统病理相关。NDRG1基因的无义突变可引起遗传性运动和感觉神经疾病，这一理论也被一些有关NDRG1能保持髓鞘和轴突存活的研究所支持[12,13]。在阿尔茨海默病中也发现NDRG2的上调表达[14]。NDRG1和 NDRG2在多个位点被Akt 和 SGK1磷酸化。但是其磷酸化的具体生理机制尚不清楚[15,16].

1. Shimono, A. et al. (1999) Mech Dev 83, 39-52.
2. Qu, X. et al. (2002) Mol Cell Biochem 229, 35-44.
3. Zhou, R.H. et al. (2001) Genomics 73, 86-97.
4. Yao, L. et al. (2008) Acta Biochim Biophys Sin (Shanghai) 40, 625-35.
5. Ellen, T.P. et al. (2008) Carcinogenesis 29, 2-8.
6. Kurdistani, S.K. et al. (1998) Cancer Res 58, 4439-44.
7. Park, H. et al. (2000) Biochem Biophys Res Commun 276, 321-8.
8. Stein, S. et al. (2004) J Biol Chem 279, 48930-40.
9. Cangul, H. (2004) BMC Genet 5, 27.
10. Wang, L. et al. (2008) Cell Physiol Biochem 21, 239-50.
11. Liu, N. et al. (2008) Nucleic Acids Res 36, 5335-49.
12. Kalaydjieva, L. et al. (2000) Am J Hum Genet 67, 47-58.
13. Okuda, T. et al. (2004) Mol Cell Biol 24, 3949-56.
14. Mitchelmore, C. et al. (2004) Neurobiol Dis 16, 48-58.
15. Murray, J.T. et al. (2004) Biochem J 384, 477-88.
16. Burchfield, J.G. et al. (2004) J Biol Chem 279, 18623-32.

原厂资料：

Specificity / Sensitivity

NDRG2 Antibody recognizes endogenous levels of total NDRG2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys25 of human NDRG2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The NDRG (N-Myc downstream-regulated gene) family, consisting of NDRG1, NDRG2, NDRG3, and NDRG4, are a group of structurally related proteins with roles in cell proliferation, differentiation, apoptosis, stress responses, and cell migration/metastasis (1-3). NDRG1 was originally identified as a protein that was up-regulated in N-Myc knockout mice (1). Proteins in the NDRG family, particularly NDRG1 and NDRG2, have been reported to be down-regulated in various cancer tissues, and have been suggested to function as a tumor suppressors (4,5).Expression of NDRG1 and NDRG2 is up-regulated by p53 and HIF-1, and contributes to apoptosis driven by those pathways (6-11). NDRG1 and NDRG2 are also associated with pathological conditions in the nervous system. Nonsense mutation of the NDRG1 gene has been shown to cause hereditary motor and sensory neuropathy-Lom (HMSNL), which is supported by studies demonstrating the role of NDRG1 in maintaining myelin sheaths and axonal survival (12, 13). Elevated expression of NDRG2 has been observed with Alzheimer's disease (14). Both NDGR1 and NDGR2 are phosphorylated at multiple sites by Akt and/or SGK1, although the precise physiological role of this phosphorylation is not known (15, 16).

1. Shimono, A. et al. (1999) Mech Dev 83, 39-52.
2. Qu, X. et al. (2002) Mol Cell Biochem 229, 35-44.
3. Zhou, R.H. et al. (2001) Genomics 73, 86-97.
4. Yao, L. et al. (2008) Acta Biochim Biophys Sin (Shanghai) 40, 625-35.
5. Ellen, T.P. et al. (2008) Carcinogenesis 29, 2-8.
6. Kurdistani, S.K. et al. (1998) Cancer Res 58, 4439-44.
7. Park, H. et al. (2000) Biochem Biophys Res Commun 276, 321-8.
8. Stein, S. et al. (2004) J Biol Chem 279, 48930-40.
9. Cangul, H. (2004) BMC Genet 5, 27.
10. Wang, L. et al. (2008) Cell Physiol Biochem 21, 239-50.
11. Liu, N. et al. (2008) Nucleic Acids Res 36, 5335-49.
12. Kalaydjieva, L. et al. (2000) Am J Hum Genet 67, 47-58.
13. Okuda, T. et al. (2004) Mol Cell Biol 24, 3949-56.
14. Mitchelmore, C. et al. (2004) Neurobiol Dis 16, 48-58.
15. Murray, J.T. et al. (2004) Biochem J 384, 477-88.
16. Burchfield, J.G. et al. (2004) J Biol Chem 279, 18623-32.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.