CNPase (D83E10) XP? Rabbit mAb

• 产品编号：CST-5664S      品牌：CST       原厂货号：5664S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CNPase (D83E10) XP® Rabbit mAb 兔单抗识别内源性的总CNPase蛋白。该单克隆抗体由合成的人类CNPase蛋白中Val81位点附近肽段免疫动物而制成。CNPase（2',3'-环核苷酸 3'-磷酸二酯酶）在体外催化水解 2',3'-环核苷酸为2'-核苷酸。该核苷酸磷酸二酯酶在体内的分子功能和自然底物仍在研究中（1）。在少突胶质细胞和雪旺氏细胞中有CNPase高表达，其表达在中枢神经系统总髓鞘蛋白中约占4％的（2）。CNPase结合到微管蛋白二聚体，在微管蛋白聚合和胶质细胞生长中发挥作用（3）。典型的髓鞘在CNPase基因敲除的小鼠可见，但它们发生包括轴突丢失的神经退行性变和轴索变性前的异常少突胶质细胞帕拉节环（paranodal loop）结构。帕拉节环通常在轴突胶质细胞信号通路中与axolemma联系；CNPase基因敲除小鼠的神经退行性变继发于细胞与细胞的通信受损（4）。

1. Esposito, C. et al. (2008) Biochemistry 47, 308-19.
2. Kozlov, G. et al. (2003) J Biol Chem 278, 46021-8.
3. Lee, J. et al. (2005) J Cell Biol 170, 661-73.
4. Lappe-Siefke, C. et al. (2003) Nat Genet 33, 366-74.

原厂资料：

Specificity / Sensitivity

CNPase (D83E10) Rabbit mAb recognizes endogenous levels of total CNPase protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val81 of human CNPase protein.

Background

CNPase (2', 3’-cyclic nucleotide 3'-phosphodiesterase) catalyzes the in vitro hydrolysis of 2’, 3’-cyclic nucleotides to produce 2’-nucleotides. The in vivo molecular function and native substrate of this nucleotide phosphodiesterase remains under investigation (1). High CNPase expression is seen in oligodendrocytes and Schwann cells as CNPase accounts for roughly 4% of the total myelin protein in the central nervous system (2). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization, and oligodendrocyte process outgrowth (3). Typical myelination is seen in CNPase knock-out mice, but they suffer severe neurodegeneration from axonal loss and oligodendrocytes display abnormal paranodal loop structure prior to axonal degeneration. Paranodal loops typically contact the axolemma in axon-glial signaling; neurodegeneration in CNPase knock-out mice is a secondary consequence of impaired cell-cell communication (4).

1. Esposito, C. et al. (2008) Biochemistry 47, 308-19.
2. Kozlov, G. et al. (2003) J Biol Chem 278, 46021-8.
3. Lee, J. et al. (2005) J Cell Biol 170, 661-73.
4. Lappe-Siefke, C. et al. (2003) Nat Genet 33, 366-74.

注意事项：

Storage: Supplied in 10mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 μg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at -20ºC. Do not aliquot the antibody