Thymidylate Synthase (TS106) Mouse mAb

• 产品编号：CST-5653S      品牌：CST       原厂货号：5653S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Thymidylate Synthase (TS106) Mouse mAb鼠单抗用于检测内源性&Thymidylate Synthase总蛋白水平。此单抗用全长的人重组胸苷酸合成酶免疫动物制备而得。在胸腺嘧啶核苷酸合成过程中，脱氧尿嘧啶的甲基化，生成脱氧胸腺嘧啶核苷酸是一个关键步骤（1,2，reviewed in 3）。此过程由胸苷酸合成酶（TS或者TYMS）催化，它是由两个30　kDa的亚基组成的同源二聚体。TS是一个胞内酶，负责催化胸苷酸的从头合成反应，因此是DNA合成过程中的必需酶，在增殖细胞中的活性最高（1）。作为脱氧胸腺嘧啶核苷酸的唯一来源，TS是抗瘤药物如5-FU的一个重要靶点（1－5）。5-FU作为TS的抑制剂，在对抗实体瘤，如结肠，乳腺，头，颈肿瘤时作用显著。研究表明，有癌转移的病人，TS的表达水平较低，对5-FU处理比较敏感，而TS表达水平较高的病人对药物不太敏感（5）。TS的表达可能有助于预测病人的总体生存情况，但TS表达对于不同类型的癌症预后的预测价值还正在研究当中（6－10）。

1. Johnston, P.G. et al. (1991) Cancer Res 51, 6668-76.
2. Aschele, C. et al. (2002) Ann Oncol 13, 1882-92.
3. Jackman, A.L. and Calvert, A.H. (1995) Ann Oncol 6, 871-81.
4. Van Triest, B. et al. (2000) J Histochem Cytochem 48, 755-60.
5. Johnston, P.G. et al. (1994) J Clin Oncol 12, 2640-7.
6. Kwon, H.C. et al. (2007) Ann Oncol 18, 504-9.
7. Allegra, C.J. et al. (2002) J Clin Oncol 20, 1735-43.
8. Allegra, C.J. et al. (2003) J Clin Oncol 21, 241-50.
9. Tsourouflis, G. et al. (2008) Dig Dis Sci 53, 1289-96.
10. Kim, S.H. et al. (2009) Am J Clin Oncol 32, 38-43.

原厂资料：

Specificity / Sensitivity

Thymidylate Synthase (TS106) Mouse mAb detects endogenous levels of total Thymidylate Synthase protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with full-length recombinant human Thymidylate Synthase protein.

Background

The methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) is an essential step in the formation of thymine nucleotides (1,2, reviewed in 3). This process is catalyzed by thymidylate synthase (TS or TYMS), a homodimer composed of two 30 kDa subunits. TS is an intracellular enzyme that provides the sole de novo source of thymidylate, making it a required enzyme in DNA biosynthesis with activity highest in proliferating cells (1). Being the exclusive source of dTMP, TS is also an important target for anticancer agents such as 5-fluorouracil (5-FU) (1-5). 5-FU acts as a TS inhibitor and is active against solid tumors such as colon, breast, head, and neck. Studies have shown that patients with metastases expressing lower levels of TS have a higher response rate to treatment with 5-FU than patients with tumors that have increased levels of TS (5). TS expression may be useful in predicting overall patient survival, but the prognostic value of TS expression continues to be a subject of investigation for different types of cancers (6-10).

1. Johnston, P.G. et al. (1991) Cancer Res 51, 6668-76.
2. Aschele, C. et al. (2002) Ann Oncol 13, 1882-92.
3. Jackman, A.L. and Calvert, A.H. (1995) Ann Oncol 6, 871-81.
4. Van Triest, B. et al. (2000) J Histochem Cytochem 48, 755-60.
5. Johnston, P.G. et al. (1994) J Clin Oncol 12, 2640-7.
6. Kwon, H.C. et al. (2007) Ann Oncol 18, 504-9.
7. Allegra, C.J. et al. (2002) J Clin Oncol 20, 1735-43.
8. Allegra, C.J. et al. (2003) J Clin Oncol 21, 241-50.
9. Tsourouflis, G. et al. (2008) Dig Dis Sci 53, 1289-96.
10. Kim, S.H. et al. (2009) Am J Clin Oncol 32, 38-43.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody