Phospho-SGK3 (Thr320) (D30E6) Rabbit mAb

• 产品编号：CST-5642S      品牌：CST       原厂货号：5642S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-SGK3 (Thr320) (D30E6) Rabbit mAb兔单抗可以检测Thr320位点被磷酸化的过表达水平的SGK3蛋白。单克隆抗体是采用人类SGK3蛋白的Thr320周围残基相对应的合成肽段免疫动物生产的。血清和糖皮质激素诱导激酶（SGK）是一个丝氨酸/苏氨酸激酶，与Akt紧密联系（1）。SGK可以被多种刺激包括血清，糖皮质激素，卵泡刺激素，渗透压冲击和盐皮质激素迅速诱导。SGK可以被肝细胞生长因子HGF刺激的PI3K依赖的通路和整合素介导的PI3K非依赖的通路激活（2,3）。SGK的诱导和激活参与激活细胞抗凋亡和细胞周期调控（4-6）。SGK在钾，钠，氯离子通道激活中也起重要作用，表明其参与调控细胞的存活，神经元的兴奋，肾钠排泄等过程（2）。SGK被泛素化和蛋白酶体降解等过程负向调控（7）。SGK1也可以调控多种靶蛋白，例如GSK-3, NEDD4, iNOS, AMPAR, PSD95, Tau, NF-κB, CREB, 和 FKHR-L1 (8,9)。SSGK3显示为一种PI3K信号通路的下游信号分子。在肿瘤中，它的激活和被PDK1在Thr320位点磷酸化可能是一种Akt依赖的信号转导（8）。

1. Webster, M.K. et al. (1993) Mol Cell Biol 13, 2031-40.
2. Kobayashi, T. and Cohen, P. (1999) Biochem J 339 ( Pt 2), 319-28.
3. Park, J. et al. (1999) EMBO J 18, 3024-33.
4. Brunet, A. et al. (2001) Mol Cell Biol 21, 952-65.
5. Mikosz, C.A. et al. (2001) J Biol Chem 276, 16649-54.
6. Hayashi, M. et al. (2001) J Biol Chem 276, 8631-4.
7. Brickley, D.R. et al. (2002) J Biol Chem 277, 43064-70.
8. Vasudevan, K.M. et al. (2009) Cancer Cell 16, 21-32.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey

Specificity / Sensitivity

Phospho-SGK3 (Thr320) (D30E6) Rabbit mAb detects overexpressed levels of SGK3 protein only when phosphorylated at Thr320.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr320 of human SGK3 protein.

Background

Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt (1). SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock, and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways (2,3). Induction and activation of SGK has been implicated in activating the modulation of anti-apoptotic and cell cycle regulation (4-6). SGK also plays an important role in activating certain potassium, sodium, and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion (2). SGK is negatively regulated by ubiquitination and proteasome degradation (7).Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt (1). SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock, and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways (2,3). Induction and activation of SGK has been implicated in activating the modulation of anti-apoptotic and cell cycle regulation (4-6). SGK also plays an important role in activating certain potassium, sodium, and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion (2). SGK is negatively regulated by ubiquitination and proteasome degradation (7).In addition to its membrane channel and carrier functions, SGK1 also regulates a broad range of targets, such as GSK-3, NEDD4, iNOS, AMPAR, PSD95, Tau, NF-κB, CREB, and FKHR-L1 (8,9).GK3 has been shown to be a downstream signaling molecule in the PI3K pathway. Its activation and phosphorylation at Thr320 by PDK1 may be an Akt-independent manner of signaling in cancer (8).

1. Webster, M.K. et al. (1993) Mol Cell Biol 13, 2031-40.
2. Kobayashi, T. and Cohen, P. (1999) Biochem J 339 ( Pt 2), 319-28.
3. Park, J. et al. (1999) EMBO J 18, 3024-33.
4. Brunet, A. et al. (2001) Mol Cell Biol 21, 952-65.
5. Mikosz, C.A. et al. (2001) J Biol Chem 276, 16649-54.
6. Hayashi, M. et al. (2001) J Biol Chem 276, 8631-4.
7. Brickley, D.R. et al. (2002) J Biol Chem 277, 43064-70.
8. Vasudevan, K.M. et al. (2009) Cancer Cell 16, 21-32.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.