Toll-like Receptor 7 (D7) Rabbit mAb

• 产品编号：CST-5632S      品牌：CST       原厂货号：5632S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Toll-like Receptor 7 (D7) Rabbit mAb兔单抗能够检测内源水平TLR7蛋白总体水平。此单克隆抗体是通过合成人源对应的TLR7 亮氨酸富集周围的肽段来免疫动物而获得。Toll样受体(TLR) 家族成员, 是因与其密切相关的果蝇Toll受体而命名的，TLR在天然免疫中发挥了极其重要的作用(1-3)。TLRs 能够识别不同病原菌中保守的结构域并介导防御反应。TLR的触发导致了NF-κB信号通路的激活并因此调控下游的免疫和炎症基因的表达。TLRs与IL-1受体家族都包含一个大约200个氨基酸的保守区域—TIR结构域。受到激活后，TLRs 与细胞质中的含有TIR区域的一系列接头蛋白作用，包括MyD88 (myeloid differentiation factor), MAL/TIRAP (MyD88-adaptor-like/TIR-associated protein), TRIF (Toll-receptor-associated activator of interferon), and TRAM (Toll-receptor-associated molecule)。这些反应导致了IRAK1 和 IRAK4的招募和激活，并与TRAF6 形成复合体激活 TAK1 和 IKK。IKK的激活导致了正常情况下截留NF-κB在细胞质中而失去活性的IκB的降解。TLR7, 8 和9 形成一个结构上类似 TLR家族的一个组别，它们定位于细胞的内体内(4-6)。TLR7 在肺，胎盘和脾中表达量最高(4)。 TLR7 能够介导对合成化合物的应答包括imidazoquinolines, 以鸟嘌呤核苷为基础的一些诱导抗病毒的化合物(7)。TLR7对ssRNA病毒做出应答并激活NF-κB信号通路从而引起 IFN 的产生 (8-10)。

1. Akira, S. (2003) J Biol Chem 278, 38105-8.
2. Beutler, B. (2004) Nature 430, 257-63.
3. Dunne, A. and O'Neill, L.A. (2003) Sci STKE 2003, re3.
4. Medzhitov, R. et al. (1997) Nature 388, 394-7.
5. Schwandner, R. et al. (1999) J Biol Chem 274, 17406-9.
6. Takeuchi, O. et al. (1999) Immunity 11, 443-51.
7. Alexopoulou, L. et al. (2001) Nature 413, 732-8.
8. Zhang, F.X. et al. (1999) J Biol Chem 274, 7611-4.
9. Horng, T. et al. (2001) Nat Immunol 2, 835-41.
10. Oshiumi, H. et al. (2003) Nat Immunol 4, 161-7.
11. Muzio, M. et al. (1997) Science 278, 1612-5.
12. Wesche, H. et al. (1997) Immunity 7, 837-47.
13. Suzuki, N. et al. (2002) Nature 416, 750-6.
14. Irie, T. et al. (2000) FEBS Lett 467, 160-4.
15. Chuang, T.H. and Ulevitch, R.J. (2000) Eur Cytokine Netw 11, 372-8.
16. Du, X. et al. (2000) Eur Cytokine Netw 11, 362-71.
17. Heil, F. et al. (2003) Eur J Immunol 33, 2987-97.
18. Hemmi, H. et al. (2002) Nat Immunol 3, 196-200.
19. Heil, F. et al. (2004) Science 303, 1526-9.
20. Diebold, S.S. et al. (2004) Science 303, 1529-31.
21. Lund, J.M. et al. (2004) Proc Natl Acad Sci USA 101, 5598-603.

原厂资料：

Specificity / Sensitivity

Toll-like Receptor 7 (D7) Rabbit mAb detects endogenous levels of total human TLR7 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the leucine-rich repeats within the human TLR7 protein.

Background

Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-3). TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the TIR domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains including MyD88 (myeloid differentiation factor), MAL/TIRAP (MyD88-adaptor-like/TIR-associated protein), TRIF (Toll-receptor-associated activator of interferon), and TRAM (Toll-receptor-associated molecule). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of IκB that normally maintains NF-κB inactivity by sequestering it in the cytoplasm.TLR7, 8 and 9 form a group of structurally related TLR family members that are are localized to intracellular endosomes (4-6). TLR7 shows highest expression in lung, placenta, and spleen (4). TLR7 mediates responses to a class of synthetic compounds, including imidazoquinolines, guanosine-based drugs that induce anti-viral responses (7). TLR7 responds to ssRNA viruses to activate NF-κB and trigger IFN production (8-10).

1. Akira, S. (2003) J Biol Chem 278, 38105-8.
2. Beutler, B. (2004) Nature 430, 257-63.
3. Dunne, A. and O'Neill, L.A. (2003) Sci STKE 2003, re3.
4. Medzhitov, R. et al. (1997) Nature 388, 394-7.
5. Schwandner, R. et al. (1999) J Biol Chem 274, 17406-9.
6. Takeuchi, O. et al. (1999) Immunity 11, 443-51.
7. Alexopoulou, L. et al. (2001) Nature 413, 732-8.
8. Zhang, F.X. et al. (1999) J Biol Chem 274, 7611-4.
9. Horng, T. et al. (2001) Nat Immunol 2, 835-41.
10. Oshiumi, H. et al. (2003) Nat Immunol 4, 161-7.
11. Muzio, M. et al. (1997) Science 278, 1612-5.
12. Wesche, H. et al. (1997) Immunity 7, 837-47.
13. Suzuki, N. et al. (2002) Nature 416, 750-6.
14. Irie, T. et al. (2000) FEBS Lett 467, 160-4.
15. Chuang, T.H. and Ulevitch, R.J. (2000) Eur Cytokine Netw 11, 372-8.
16. Du, X. et al. (2000) Eur Cytokine Netw 11, 362-71.
17. Heil, F. et al. (2003) Eur J Immunol 33, 2987-97.
18. Hemmi, H. et al. (2002) Nat Immunol 3, 196-200.
19. Heil, F. et al. (2004) Science 303, 1526-9.
20. Diebold, S.S. et al. (2004) Science 303, 1529-31.
21. Lund, J.M. et al. (2004) Proc Natl Acad Sci USA 101, 5598-603.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody