ARP2 (D85D5) Rabbit mAb

• 产品编号：CST-5614S      品牌：CST       原厂货号：5614S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ARP2 (D85D5)Rabbit mAb可以识别内源性总的ARP2蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人ARP2蛋白羧基末端附近氨基酸一致。Actin 成核作用——从已有的细丝形成新的肌动蛋白丝，会在细胞运动，分裂和细胞内示踪过程中影响肌动蛋白丝的结构。一个重要的actin成核作用蛋白复合物是高度保守的ARP2/3蛋白，由ARP2, ARP3,和 ARPC1-5组成。在激活了成核作用促进因子，类似WASP/WAVE 或 cortactin 后，ARP2/3复合物促进现有肌动蛋白丝形成分支以及姐妹肌动蛋白丝（1）。伪足的形成，降解胞外基质形成的小的细胞结构，能够被ARP2/3增强。ARP2/3与caldesmon竞争，caldesmon是一个肌动蛋白丝结合蛋白，负向的调控podosome形成（2）。与N-WASP一样，ARP2/3复合物能够调控核肌动蛋白丝的成核作用并控制肌动蛋白丝在转录期间的多聚化（3）。

（3）。

1. Goley, E.D. and Welch, M.D. (2006) Nat. Rev. Mol. Cell Biol. 7, 713-726.
2. Morita, T. et al. (2007) J. Biol. Chem. 282, 8454-8463.
3. Yoo, Y. et al. (2007) J. Biol. Chem. 282, 7616-7623.

原厂资料：

Specificity / Sensitivity

ARP2 (D85D5) Rabbit mAb recognizes endogenous levels of total ARP2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ARP2 protein.

Background

Actin nucleation, the formation of new actin filaments from existing filaments, affects actin filament structure during cell motility, division, and intracellular trafficking. An important actin nucleation protein complex is the highly conserved ARP2/3 complex, consisting of ARP2, ARP3, and ARPC1-5. The ARP2/3 complex promotes branching of an existing actin filament and formation of a daughter filament following activation by nucleation-promoting factors, such as WASP/WAVE or cortactin (1). The formation of podosomes, small cellular projections that degrade the extracellular matrix, is enhanced by ARP2/3 complex action. ARP2/3 competes with caldesmon, an actin binding protein shown to negatively affect podosome formation (2). Along with N-WASP, the ARP2/3 complex regulates nuclear actin filament nucleation and controls actin polymerization during transcription (3).

（3）。

1. Goley, E.D. and Welch, M.D. (2006) Nat. Rev. Mol. Cell Biol. 7, 713-726.
2. Morita, T. et al. (2007) J. Biol. Chem. 282, 8454-8463.
3. Yoo, Y. et al. (2007) J. Biol. Chem. 282, 7616-7623.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.