BACE (D10E5) Rabbit mAb

• 产品编号：CST-5606S      品牌：CST       原厂货号：5606S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

BACE (D10E5) Rabbit mAb 兔单抗识别内源性的总BACE蛋白。该单克隆抗体由合成的人类BACE蛋白中Asp490位点附近肽段免疫动物而制成。β-分泌酶（BACE）是天门冬氨酸蛋白酶，它催化APP处理的初始步骤，它裂解并释放出可溶性的APPβ（sAPPβ）胞外段至细胞外，并产生包含99个氨基酸，以羧基为末端的膜结合片段（CTF99）。γ-分泌酶对CTF99额外的处理产生了在阿尔茨海默病（AD）患者中脑内沉积的淀粉样蛋白Aβ。BACE的抑制剂是AD治疗中有吸引力对象，因为它在淀粉样APP处理的第一步和限速步骤中都有催化作用（1）。BACE1前体在内质网中合成，然后转运到高尔基体反面中进行成熟（2）。BACE1以二聚体发挥功能，它的Ser498位点被酪蛋白激酶1（casein kinase 1）磷酸化，对于从早期内体中回收至细胞膜上是必要的。

1. Hunt, C.E. and Turner, A.J. (2009) FEBS J 276, 1845-59.
2. Benjannet, S. et al. (2001) J Biol Chem 276, 10879-87.
3. Walter, J. et al. (2001) J Biol Chem 276, 14634-41.
4. He, X. et al. (2005) J Biol Chem 280, 11696-703.

原厂资料：

Specificity / Sensitivity

BACE (D10E5) Rabbit mAb detects endogenous levels of total BACE protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp490 of human BACE protein.

Background

β-secretase (BACE) is an aspartic acid proteinase that catalyses the initial step in APP processing by cleaving and releasing a soluble, extracellular APP-β (sAPPβ) ectodomain and generating a membrane-bound, carboxy-terminal fragment consisting of 99 amino acids (CTF99). Additional processing of CTF99 by γ-secretase generates the amyloid β-peptide (Aβ) that forms aggregates in the brains of Alzheimer disease (AD) patients. BACE is an attractive target for inhibitors in AD therapy since it catalyses the first and rate limiting step in amyloidogenic APP processing (1). Pro-BACE-1 is synthesized in the ER before it is transported to the trans-Golgi network to undergo maturation (2). BACE-1 functions as a dimer and is phosphorylated at Ser498 by casein kinase 1, which is necessary for recycling back to the membrane from the early endosome (3,4).

1. Hunt, C.E. and Turner, A.J. (2009) FEBS J 276, 1845-59.
2. Benjannet, S. et al. (2001) J Biol Chem 276, 10879-87.
3. Walter, J. et al. (2001) J Biol Chem 276, 14634-41.
4. He, X. et al. (2005) J Biol Chem 280, 11696-703.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody