Anti-biotin (D5A7) Rabbit mAb (HRP Conjugate)

• 产品编号：CST-5571S      品牌：CST       原厂货号：5571S
• 产品分类：抗体 > 一抗 > 酶标记一抗 > 辣根过氧化物酶（HRP）
• 应用分类：

描述：

抗生物素（D5A7）兔源当克隆抗体（HRP标记）可以识别与蛋白、肽、寡核苷酸或固相基质上的生物素。抗生物素（D5A7）兔源抗体（HRP标记）可以与辣根过氧化物酶通过气氨基基团与碳水化合物基团结合。化学反光检测已经成为western blots检测中最好和最全面的检测方法。它们减少了放射性物质和有毒显色基质相关的危害。这些方法的检测速度和灵敏度的都是传统替代品无可比拟的。因为结果生成在胶片上，因此可以永久记录和保存实验结果，而且用化学发光方法检测出的印迹可以很容易被洗脱并且易于与采用其它抗体重新检测。辣根过氧化物酶（HRP）结合的二抗与化学发光的底物特异性结合生成光信号。辣根过氧化物酶与抗体的轭合物有非常高的周转率，在较短的时间里有很高的敏感性。

原厂资料：

Specificity / Sensitivity

Anti-biotin (D5A7) Rabbit mAb (HRP Conjugate) recognizes biotin attached to proteins, peptides, oligonucleotides and solide matrices.

Description

Anti-biotin (D5A7) Rabbit mAb is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups.

Applications

This product has been optimized to detect biotinylated primary antibodies.

Background

Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 2 mg/ml bovine serum albumin (BSA) and 50% glycerol.
Store at –20°C.