DJ-1 (D21E11) Rabbit mAb

• 产品编号：CST-5560S      品牌：CST       原厂货号：5560S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DJ-1 (D21E11)Rabbit mAb 兔单抗识别内源性的总DJ-1蛋白。推荐使用DJ-1 (D21E11)Rabbit mAb 兔单抗来检测人类样本，但也可以用来检测小鼠和大鼠的样本。该单克隆抗体由合成的人类DJ-1蛋白Gly150位点附近肽段免疫动物而制成。帕金森病（PD）的特点是路易体（细胞内含体）的存在和多巴胺能神经元的丢失。α-synuclein, Parkin 和 DJ-1 的基因变异与帕金森病有关。α-synuclein 是路易小体中的主要沉积物。Parkin与泛素蛋白途径的蛋白降解有关，其突变可以造成早发的PD（1）。DJ-1的功能丧失突变可以导致早发性帕金森病，但DJ-1与多种功能有关：它与RAS合作，增加细胞转化，它正向调节雄激素受体的转录，它可能作为一种氧化应激指标（2-5）。多巴胺D2受体介导的功能在DJ-1(-/-)的小鼠中减少，导致长期抑郁（6）。

1. Borrelli, E. (2005) Neuron 45, 479-81.
2. Bonifati, V. et al. (2003) Science 299, 256-9.
3. Nagakubo, D. et al. (1997) Biochem. Biophys. Res. Commun. 231, 509-13.
4. Takahashi, K. et al. (2001) J. Biol. Chem. 276, 37556-63.
5. Mitsumoto, A. and Nakagawa, Y. (2001) Free Radic. Res. 35, 885-93.
6. Goldberg, M.S. et al. (2005) Neuron 45, 489-96.

原厂资料：

Specificity / Sensitivity

DJ-1 (D21E11) Rabbit mAb detects endogenous levels of total DJ-1 protein. DJ-1 (D21E11) Rabbit mAb is recommended to detect human samples but may also detect mouse and rat samples weakly.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly150 of human DJ-1 protein.

Background

Parkinson's disease (PD) is characterized by the presence of Lewy bodies (intracellular inclusions) and by the loss of dopaminergic neurons. Mutations in α-synuclein, Parkin and DJ-1 have linked these genes to PD. α-synuclein is a major component of the aggregates found in Lewy bodies. Parkin is involved in protein degradation through the ubiquitin-proteasome pathway, and mutations in Parkin cause early onset of PD (1). Loss-of-function mutations in DJ-1 cause early onset of PD, but DJ-1 is associated with multiple functions: it cooperates with Ras to increase cell transformation, it positively regulates transcription of the androgen receptor, and it may function as an indicator of oxidative stress (2-5). Dopamine D2 receptor-mediated functions are greatly impaired in DJ-1 (-/-) mice resulting in reduced long-term depression (6).

1. Borrelli, E. (2005) Neuron 45, 479-81.
2. Bonifati, V. et al. (2003) Science 299, 256-9.
3. Nagakubo, D. et al. (1997) Biochem. Biophys. Res. Commun. 231, 509-13.
4. Takahashi, K. et al. (2001) J. Biol. Chem. 276, 37556-63.
5. Mitsumoto, A. and Nakagawa, Y. (2001) Free Radic. Res. 35, 885-93.
6. Goldberg, M.S. et al. (2005) Neuron 45, 489-96.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody