DDX4 (2F9H5) Mouse mAb

• 产品编号：CST-5555S      品牌：CST       原厂货号：5555S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DDX4 (2F9H5) Mouse mAb检测内源性的DDX4总蛋白水平。使用纯化重组的人源DDX4蛋白去免疫动物从而制备单克隆抗体。DDX4是一个ATP依赖的DEAD-box RNA解旋酶，其在生殖细胞的拟染色体中被发现(1)。这种解旋酶是生殖细胞系中特异的酶，并对于生殖细胞的生长是必须的(2)。研究证明小鼠的DDX4蛋白能和Dicer蛋白相互作用，从而说明该蛋白对microRNA介导RNA沉默起到一定作用(1)。

1. Kotaja, N. et al. (2006) Proc Natl Acad Sci USA 103, 2647-52.
2. Albamonte, M.S. et al. (2008) Hum Reprod 23, 1895-901.

原厂资料：

Specificity / Sensitivity

DDX4 (2F9H5) Mouse mAb detects endogenous levels of total DDX4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a purified recombinant fragment of human DDX4.

Background

DDX4 is an ATP-dependent DEAD-box RNA helicase found in the chromatoid body of the germ cells (1). This enzyme is specific to germ cells and is necessary for germ cell development (2). Mouse DDX4 was shown to interact with Dicer suggesting its role in microRNA-mediated RNA silencing (1).

1. Kotaja, N. et al. (2006) Proc Natl Acad Sci USA 103, 2647-52.
2. Albamonte, M.S. et al. (2008) Hum Reprod 23, 1895-901.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody