Phospho-PZR (Tyr263) Antibody

• 产品编号：CST-5543S      品牌：CST       原厂货号：5543S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-PZR (Tyr263)抗体仅可以识别263酪氨酸磷酸化后后的PZR蛋白。抗体与其他酪氨酸激酶蛋白没有交叉反应。合成对应人PZR蛋白263位酪氨酸及其邻近氨基酸残基序列的多肽免疫动物获得多克隆抗体。PZR（蛋白zero相关）是一个免疫球超家族蛋白可以通过其细胞内免疫受体：基于酪氨酸的抑制基序（ITIMs）与酪氨酸磷酸酯酶SHP-2特异性结合（1,2）。PZR可以被c-Src, c-Fyn, c-Lyn, Csk, 和c-Abl磷酸化（3）。PP1，一个Src家族激酶抑制子，能够抑制PZR磷酸化（4,5）。PZR有3个剪切异构体，分别命名为PZR, PZRa, and PZRb;PZRa和PZRb都缺乏ITIMs（6,7）。PZR是刀豆素A的主要受体在细胞信号传导中通过c-Src发挥重要作用（4）。PZR在多种细胞中表达，位于BAECs和间皮瘤（REN）细胞连接和细胞内颗粒中。PZR被认为是一种细胞粘附分子，可能在SHP-2-依赖的信号通路中涉及内皮细胞间连接（3）。Noonan 综合征小鼠模型的胚胎发育时期可以观察到PZR酪氨酸高度磷酸化（8）。

1. Zhao, Z.J. and Zhao, R. (1998) J Biol Chem 273, 29367-72.
2. Zhao, R. and Zhao, Z.J. (2000) J Biol Chem 275, 5453-9.
3. Kusano, K. et al. Endothelium 15, 127-36.
4. Zhao, R. et al. (2002) J Biol Chem 277, 7882-8.
5. Zhao, R. et al. (2003) J Biol Chem 278, 42893-8.
6. Zannettino, A.C. et al. (2003) Biochem J 370, 537-49.
7. Zhao, R. and Zhao, Z.J. (2003) Biochem Biophys Res Commun 303, 1028-33.
8. Eminaga, S. and Bennett, A.M. (2008) J Biol Chem 283, 15328-38.

原厂资料：

Specificity / Sensitivity

Phospho-PZR (Tyr263) Antibody recognizes endogenous levels of PZR protein only when phosphorylated at Tyr263. This antibody does not cross-react with other tyrosine kinase proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr263 of human PZR protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

PZR (Protein zero related) is an immunoglobulin superfamily protein that specifically binds the tyrosine phosphatase SHP-2 through its intracellular immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (1,2). PZR is phosphorylated by c-Src, c-Fyn, c-Lyn, Csk, and c-Abl (3). PP1, a Src family kinase inhibitor, inhibits PZR phosphorylation (4,5). There are three alternatively spliced isoforms, designated as PZR, PZRa, and PZRb; both PZRa and PZRb lack ITIMs (6,7). PZR is the main receptor of ConA and has an important role in cell signaling via c-Src (4). PZR is expressed in many cell types and is localized to cell contacts and intracellular granules in BAECs and mesothelioma (REN) cells. PZR has been implicated as a cell adhesion protein that may be involved in SHP-2-dependent signaling at interendothelial cell contacts (3). Hypertyrosine phosphorylation of PZR was observed during embryogenesis in a mouse model of Noonan syndrome (8).

1. Zhao, Z.J. and Zhao, R. (1998) J Biol Chem 273, 29367-72.
2. Zhao, R. and Zhao, Z.J. (2000) J Biol Chem 275, 5453-9.
3. Kusano, K. et al. Endothelium 15, 127-36.
4. Zhao, R. et al. (2002) J Biol Chem 277, 7882-8.
5. Zhao, R. et al. (2003) J Biol Chem 278, 42893-8.
6. Zannettino, A.C. et al. (2003) Biochem J 370, 537-49.
7. Zhao, R. and Zhao, Z.J. (2003) Biochem Biophys Res Commun 303, 1028-33.
8. Eminaga, S. and Bennett, A.M. (2008) J Biol Chem 283, 15328-38.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.