Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb (Sepharose^? Bead Conjuga

• 产品编号：CST-5501S      品牌：CST       原厂货号：5501S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb (Sepharose Bead Conjugate)兔单抗能够检测PXS*P或S*PXR/K基序，以及PXS*PXR/K基序中的磷酸化丝氨酸。此抗体是磷酸化特异性的，与包含磷酸化苏氨酸或磷酸化酪氨酸的肽段或蛋白无交叉反应。（美国专利号：6,441,140、6,982,318、7,259,022、7,344,714；U.S.S.N. 11,484,485；及其国外相应专利）该单克隆抗体用合成的磷酸化MAPK/CDK底物多肽免疫动物制备。该CST抗体是通过主要的氨基酸集团共价结合N-羟基丁二酰亚胺（NHS）活化的琼脂糖珠进行固定的。Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb (Sepharose Bead Conjugate)兔单抗对于Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K)的免疫沉淀非常有用。该抗体与未偶联的Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb兔单抗 #2325具有相同的种属交叉反应性。MAPK和CDK丝氨酸/苏氨酸蛋白激酶家族在细胞增殖和周期控制中扮演重要角色。这些激酶磷酸化脯氨酸残基前的苏氨酸或丝氨酸（1-3）。MAPK磷酸化含有共有序列PX(S/T)P的底物，CDKs磷酸化含有共有序列(S/T)PXK/R的底物。CST公司开发了可以结合脯氨酸残基前的磷酸化苏氨酸、基序为PXS*/T*P和或S*PXK/R的抗体，用以研究和发现新的MAPK和CDK底物（4,5）。

1. Cross, T.G. et al. (2000) Exp Cell Res 256, 34-41.
2. Reynolds, C.H. et al. (2000) J Neurochem 74, 1587-95.
3. Seger, R. and Krebs, E.G. (1995) FASEB J 9, 726-35.
4. Holmes, J.K. and Solomon, M.J. (1996) J Biol Chem 271, 25240-6.
5. Songyang, Z. et al. (1996) Mol Cell Biol 16, 6486-93.

原厂资料：

Specificity / Sensitivity

Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb (Sepharose Bead Conjugate) detects phospho-serine in a PXS*P or S*PXR/K motif, as well as a PXS*PXR/K motif. The antibody is phospho-specific, and does not react with phospho-threonine- or phospho-tyrosine-containing peptides/proteins. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic phospho-MAPK/CDK substrate peptides.

Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb (Sepharose Bead Conjugate) is useful for the immunoprecipitation of Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K). The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb #2325.

Background

The MAPK and CDK families of serine/threonine protein kinases play important roles in proliferation and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-3). MAPK phosphorylates substrates with the consensus sequence PX(S/T)P, and CDKs phosphorylate substrates containing the consensus sequence (S/T)PXR/K. Cell Signaling Technology has developed antibodies that bind to phospho-threonine followed by proline, motifs PXS*/T*P and/or S*PXR/K, for use in the study and discovery of new MAPK and CDK substrates (4,5).

1. Cross, T.G. et al. (2000) Exp Cell Res 256, 34-41.
2. Reynolds, C.H. et al. (2000) J Neurochem 74, 1587-95.
3. Seger, R. and Krebs, E.G. (1995) FASEB J 9, 726-35.
4. Holmes, J.K. and Solomon, M.J. (1996) J Biol Chem 271, 25240-6.
5. Songyang, Z. et al. (1996) Mol Cell Biol 16, 6486-93.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not
aliquot the antibody.