GST (91G1) Rabbit mAb (HRP Conjugate)

• 产品编号：CST-5475S      品牌：CST       原厂货号：5475S
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

该抗体可以检测转染的GST融合蛋白。单克隆抗体是通过用GST融合蛋白免疫动物生产的。此抗体通过辣根过氧化物酶的氨基基团与碳水化合物基团结合，此种HRP标记的抗体与未标记的GST (91G1) Rabbit mAb #2625用来检测同种交叉反应。在免疫印迹、免疫沉淀和免疫染色等技术中，表位标签用于标记和检测蛋白。由于它们的体积很小，它们一般不会影响标记蛋白的生化特性。谷胱甘肽巯基转移酶（GST）是一种广泛应用的融合蛋白伴侣，因为它可以同时提供易于检测的Tag标签和简单的纯化过程，而对目的蛋白的生物功能几乎没有影响。在过去的十年里，大量担带有Tag标签的载体应用于原核和真核系统。

1. Guan, K.L. and Dixon, J.E. (1991) Anal Biochem 192, 262-7.
2. Davies, A.H. et al. (1993) Biotechnology (N Y) 11, 933-6.
3. Yu, J. et al. (1998) Mol Cell Biol 18, 1379-87.

原厂资料：

Specificity / Sensitivity

GST (91G1) Rabbit mAb (HRP Conjugate) detects transfected GST fusion proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a GST fusion protein.

Description

This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated GST (91G1) Rabbit mAb #2625.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).

1. Guan, K.L. and Dixon, J.E. (1991) Anal Biochem 192, 262-7.
2. Davies, A.H. et al. (1993) Biotechnology (N Y) 11, 933-6.
3. Yu, J. et al. (1998) Mol Cell Biol 18, 1379-87.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA and 50%
glycerol. Store at -20ºC. Do not aliquot the antibody.