BRSK2 (D29B6) Rabbit mAb

• 产品编号：CST-5460S      品牌：CST       原厂货号：5460S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

BRSK2 (D29B6) Rabbit mAb 兔单抗识别内源性的总BRSK2蛋白。该单克隆抗体由合成的人类BRSK2蛋白氨基末端附近肽段免疫动物而制成。脑特异性激酶1（BRSK1；SAD-B）和脑特异性激酶2（BRSK2；SAD-A）是与AMPK密切相关的丝氨酸/苏氨酸激酶。LKB1的磷酸化BRSK1T型环中的Thr189和BRSK2T型环中的Thr174，导致激酶的活化（1）。BRSK1定位在海马和小脑的突触小泡，与蛋白质Bassoon和CAST的活性区域一起，BRSK1磷酸化蛋白质RIM1的活性区域（2）。从BRSK1中进行一种选择性剪接，可在细胞周期中显示出独特的活性，它磷酸化γ-微管蛋白的Ser131位点，控制中心体复制（3）。神经元极化，包括轴突的形成，是正常大脑发育基本步骤。BRSK1 -/-和BRSK2 -/- 的小鼠在神经极化和皮层生成方面受损。体外敲除BRSK1和BRSK2会削弱轴突生长（5）。

1. Bright, N.J. et al. (2008) J Biol Chem 283, 14946-54.
2. Inoue, E. et al. (2006) Neuron 50, 261-75.
3. Alvarado-Kristensson, M. et al. (2009) Nat Cell Biol 11, 1081-92.
4. Kishi, M. et al. (2005) Science 307, 929-32.
5. Choi, Y.J. et al. (2008) Genes Dev 22, 2485-95.

原厂资料：

Specificity / Sensitivity

BRSK2 (D29B6) Rabbit mAb detects endogenous levels of total BRSK2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human BRSK2 protein.

Background

Brain-specific kinase 1 (BRSK1; SAD-B) and Brain-specific kinase 2 (BRSK2; SAD-A) are serine/threonine kinases closely related to AMPK. LKB1 phosphorylates Thr189 in the T-loop of BRSK1 and Thr174 in the T-loop of BRSK2, resulting in activation of the kinases (1). BRSK1 localizes to synaptic vesicles in the hippocampus and cerebellum, together with the active zone proteins Bassoon and CAST, and BRSK1 phoshorylates the active zone protein RIM1 (2). An alternatively spliced from of BRSK1 displays unique activity during the cell cycle, phosphorylating Ser131 of γ-tubulin and controling centrosome duplication (3). Neuronal polarization, including axon formation, is fundamental for normal brain development. BRSK1 -/- and BRSK2 -/- mice have defects in neuronal polarity and impaired corticogenesis (4). Knockdown of BRSK1 and BRSK2 in vitro diminishes axonal growth (5).

1. Bright, N.J. et al. (2008) J Biol Chem 283, 14946-54.
2. Inoue, E. et al. (2006) Neuron 50, 261-75.
3. Alvarado-Kristensson, M. et al. (2009) Nat Cell Biol 11, 1081-92.
4. Kishi, M. et al. (2005) Science 307, 929-32.
5. Choi, Y.J. et al. (2008) Genes Dev 22, 2485-95.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.