RBPSUH Antibody

• 产品编号：CST-5442S      品牌：CST       原厂货号：5442S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

RBPSUH抗体识别内源性的RBPSUH总蛋白。多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源RBPSUH蛋白的Glu111氨基酸残基序列一致，抗体由蛋白A和肽段亲和层析技术纯化得到。RBPSUH（无毛基因重组结合蛋白抑制因子）是Notch信号调控的转录复合物中的DNA结合元件。Notch未激活时，RBPSUH通过与包含组蛋白乙酰化酶的共抑制复合物相互作用抑制靶基因表达。激活时，Notch细胞内结构域（NICD）进入核内与RBPSUH结合替代共抑制复合物，并代替转录激活复合物包括Maml, 组蛋白乙酰化酶 p300，导致了Notch靶基因的转录[1-3]。另外，RBPSUH也是Epstein-Barr(EBV)病毒核内抗原2(EBNA2)的DNA结合伴侣，EBNA2对于潜伏性病毒转录和EBV感染的B细胞永生很关键[4,5]。

1. Ehebauer, M. et al. (2006) Sci STKE 2006, cm7.
2. Borggrefe, T. and Oswald, F. (2009) Cell Mol Life Sci 66, 1631-46.
3. Kopan, R. and Ilagan, M.X. (2009) Cell 137, 216-33.
4. Henkel, T. et al. (1994) Science 265, 92-5.
5. Strobl, L.J. et al. (1997) Immunobiology 198, 299-306.

原厂资料：

Specificity / Sensitivity

RBPSUH Antibody detects endogenous level of total RBPSUH protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu111 of human RBPSUH protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

RBPSUH (Recombining binding protein suppressor of hairless) is the DNA-binding component of the transcription complex regulated by Notch signaling. In the absence of Notch activation, RBPSUH suppresses target gene expression through interactions with a co-repressor complex containing histone deacetylase. Upon activation, the Notch intracellular domain (NICD) enters the nucleus and binds to RBPSUH to displace the co-repressor complex and replace with a transcription activation complex including Maml, histone acetylase p300, leading to the transcription of Notch target genes (1-3). In addition, RBPSUH is also the DNA-binding partner for Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2), a protein critical for latent viral transcription and immortalization of EBV-infected B cells (4,5).

1. Ehebauer, M. et al. (2006) Sci STKE 2006, cm7.
2. Borggrefe, T. and Oswald, F. (2009) Cell Mol Life Sci 66, 1631-46.
3. Kopan, R. and Ilagan, M.X. (2009) Cell 137, 216-33.
4. Henkel, T. et al. (1994) Science 265, 92-5.
5. Strobl, L.J. et al. (1997) Immunobiology 198, 299-306.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.