Emerin (D9B3) Rabbit mAb

• 产品编号：CST-5430S      品牌：CST       原厂货号：5430S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Emerin (D9B3) Rabbit mAb兔单抗检测内源性Emerin总蛋白。通过人工合成人源Emerin蛋白氨基末端周围相应的多肽片段去免疫动物从而制备出单克隆抗体。Emerin蛋白是一个广泛表达的核内膜的整合蛋白(1)。它包含一个LEM结构域，并且结合到数个核蛋白例如BAF (barrier-to-autointegration factor)和 A-和B-型lamins蛋白，该蛋白对细胞核功能起着重要作用(2-5)。Emerin蛋白可能通过结合其它的转录调节因子调节基因表达(6,7)。Emerin蛋白结合到β-catenin，并且抑制它的细胞核堆积(8)。最近研究证明Emerin蛋白对HIV-1的传染性是需要的(9)。Emerin蛋白的突变是Emery-Dreifuss muscular dystrophy (EDMD)的主要成因，EDMD是一种以进程性骨骼肌肉减弱为特征的紊乱 (10)。

1. Nagano, A. et al. (1996) Nat. Genet. 12, 254-259.
2. Manilal, S. et al. (1998) Biochem. Biophys. Res. Commun. 249, 643-647.
3. Clements, L. et al. (2000) Biochem. Biophys. Res. Commun. 267, 709-714.
4. Lee, K.K. et al. (2001) J. Cell Sci. 114, 4567-4573.
5. Bengtsson, L. and Wilson, K.L. (2006) Mol. Biol. Cell 17, 1154-1163.
6. Holaska, J.M. et al. (2003) J. Biol. Chem. 278, 6969-6975.
7. Haraguchi, T. et al. (2004) Eur. J. Biochem. 271, 1035-1045.
8. Markiewicz, E. et al. (2006) EMBO J. 25, 3275-3285.
9. Jacque, J.M. and Stevenson, M. (2006) Nature 441, 641-645.
10. Holaska, J.M. and Wilson, K.L. (2006) Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 288, 676-680.

原厂资料：

Specificity / Sensitivity

Emerin (D9B3) Rabbit mAb detects endogenous level of total Emerin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Emerin protein.

Background

Emerin is a broadly expressed integral protein of the nuclear inner membrane (1). It contains a LEM domain and binds to several nuclear proteins, such as BAF (barrier-to-autointegration factor) and A- and B-type lamins, which are important in nuclear functions (2-5). Emerin may regulate gene expression through binding to other transcriptional regulators (6,7). Emerin binds to β-catenin and inhibits its nuclear accumulation (8). Recent studies demonstrate that Emerin is required for HIV-1 infectivity (9). Mutations in Emerin are a major cause of Emery-Dreifuss muscular dystrophy (EDMD), a disorder characterized by progressive skeletal muscle weakening (10).

1. Nagano, A. et al. (1996) Nat. Genet. 12, 254-259.
2. Manilal, S. et al. (1998) Biochem. Biophys. Res. Commun. 249, 643-647.
3. Clements, L. et al. (2000) Biochem. Biophys. Res. Commun. 267, 709-714.
4. Lee, K.K. et al. (2001) J. Cell Sci. 114, 4567-4573.
5. Bengtsson, L. and Wilson, K.L. (2006) Mol. Biol. Cell 17, 1154-1163.
6. Holaska, J.M. et al. (2003) J. Biol. Chem. 278, 6969-6975.
7. Haraguchi, T. et al. (2004) Eur. J. Biochem. 271, 1035-1045.
8. Markiewicz, E. et al. (2006) EMBO J. 25, 3275-3285.
9. Jacque, J.M. and Stevenson, M. (2006) Nature 441, 641-645.
10. Holaska, J.M. and Wilson, K.L. (2006) Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 288, 676-680.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.