OGT Antibody

• 产品编号：CST-5368S      品牌：Cell Signaling Technology       原厂货号：5368S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

OGT抗体识别内源性的OGT总蛋白水平。该多克隆抗体用与人类OGT中Ala686位点附近的氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白A和肽亲和层析纯化而得。O-GlcNAcylatio是一个翻译后修饰,β-D-N-acetylglucosamine (GlcNAc)被共价连接到细胞质和细胞核蛋白的丝氨酸或苏氨酸残基（1,2）。这种修饰对很多细胞过程都很重要，包括代谢、细胞生长和形态、细胞凋亡和转录（2,3），研究显示它的这些修饰与癌症相关（1）。O-GlcNAc的可逆的蛋白修饰作用，作为可能的营养和压力感受器，由两个高度保守的酶催化，O-GlcNAc转移酶（OGT）和O-GlcNAcase（OGA）（4）。

1. Comer, F.I. et al. (2001) Anal Biochem 293, 169-77.
2. Slawson, C. and Hart, G.W. (2011) Nat Rev Cancer 11, 678-84.
3. Capotosti, F. et al. (2011) Cell 144, 376-88.
4. Hart, G.W. et al. (2007) Nature 446, 1017-22.

原厂资料：

Specificity / Sensitivity

OGT Antibody recognizes endogenous levels of total OGT protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala686 of human OGT protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

O-GlcNAcylation is a post-translational modification where β-D-N-acetylglucosamine (GlcNAc) is covalently linked to cytoplasmic and nuclear proteins at serine or threonine residues (1,2). This modification is important in many cellular processes including metabolism, cell growth and morphogenesis, apoptosis, and transcription (2,3), and research studies have implicated this modification in cancer (1). The reversible protein modification by O-GlcNAc, which has been suggested to be a nutrient and stress sensor, is catalyzed by two highly conserved enzymes, O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) (4).

1. Comer, F.I. et al. (2001) Anal Biochem 293, 169-77.
2. Slawson, C. and Hart, G.W. (2011) Nat Rev Cancer 11, 678-84.
3. Capotosti, F. et al. (2011) Cell 144, 376-88.
4. Hart, G.W. et al. (2007) Nature 446, 1017-22.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.