EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb (Biotinylated)

• 产品编号：CST-5354S      品牌：CST       原厂货号：5354S
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

EGFR(L858R 特异突变)的兔源单克隆抗体(43B2) (生物素化)能检测内源L858R 位点突变的EGFR蛋白的水平。为获得最佳特异性本抗体需要仔细滴定。单克隆抗体通过免疫动物得到，作为免疫源的合成多肽根据人EGFR蛋白L858R位点突变序列合成。本CST公司生产的抗体在最佳条件下与生物素偶联。该生物素偶联的抗体具有和未偶联生物素的抗EGFR(L858R 特异突变)的兔源单克隆抗体(43B2) #3197相同的物种交叉反应特性。表皮生长因子（EGF）受体是一个属于HER/ErbB蛋白家族的跨膜酪氨酸激酶，大小170kDa。EGFR酪氨酸激酶区域的体细胞突变在肺腺癌的亚分类中有发现(1-3)。其中两种突变大约占了所有突变类型的90%：一个是发生在21外显子上L858R位点的点突变，另一个是19外显子内部的短片段缺失(4,5)。19外显子异常虽然有多种形式，但常见的是E746-A750的缺失。

1. Lynch, T.J. et al. (2004) N Engl J Med 350, 2129-39.
2. Pao, W. et al. (2004) Proc Natl Acad Sci USA 101, 13306-11.
3. Haber, D.A. et al. (2005) Cold Spring Harb Symp Quant Biol 70, 419-26.
4. Kosaka, T. et al. (2004) Cancer Res 64, 8919-23.
5. Riely, G.J. et al. (2006) Clin Cancer Res 12, 7232-41.

原厂资料：

Specificity / Sensitivity

EGFR (L858R Mutant Specific) (43B2) Rabbit mAb (Biotinylated) detects endogenous levels of EGFR mutant L858R protein. Careful titration of this antibody may be required to obtain optimal specificity.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to L858R mutant sequence of human EGF receptor. 单克隆抗体通过免疫动物得到，作为免疫源的合成多肽根据人EGFR蛋白L858R位点突变序列合成。

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated EGFR (L858R Mutant Specific) (43B2) Rabbit mAb #3197. 

Background

The epidermal growth factor (EGF) receptor is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Somatic mutations in the tyrosine kinase domain of EGF receptor (EGFR) are present in a subset of lung adenocarinomas that respond to EGFR inhibitors such as gefinitib and erlotinib (1-3). Two types of mutations account for approximately 90% of mutated cases: a specific point mutation, L858R, that occurs in exon 21 and short in-frame deletions in exon 19 (4,5). The most frequent exon 19 deletion is E746-A750, accounting for 90% of lesions at this site, although some rare variants occur.

1. Lynch, T.J. et al. (2004) N Engl J Med 350, 2129-39.
2. Pao, W. et al. (2004) Proc Natl Acad Sci USA 101, 13306-11.
3. Haber, D.A. et al. (2005) Cold Spring Harb Symp Quant Biol 70, 419-26.
4. Kosaka, T. et al. (2004) Cancer Res 64, 8919-23.
5. Riely, G.J. et al. (2006) Clin Cancer Res 12, 7232-41.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50%
glycerol. Store at –20°C. Do not aliquot the antibodies.