hnRNP Q/R Antibody

• 产品编号：CST-5351S      品牌：CST       原厂货号：5351S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

hnRNP Q/R Antibody检测内源性hnRNP Q和hnRNP R总蛋白水平。通过人工合成人源hnRNP Q蛋白羧基末端序列相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Heterogeneous nuclear ribonucleoprotein Q和R属于hnRNP蛋白家族，这个家族涉及到RNA结合、RNA生物合成和mRNA从细胞核到细胞质的转运(1-3)。这两个蛋白质由不同的基因编码，但是有83%的同源性。hnRNP Q蛋白有三个选择性剪接的变体 (hnRNP Q1-3)(1-3)。羧基末端精氨酸残基的甲基化对于核定位是必须的(4)。hnRNP Q和AUF1结合到富含AU的mRNA并且调节mRNA降解(5)。hnRNP Q异构体在介导survival of motor neuron(SMN) 复合物 (6,7) 的细胞核功能、调整RNA生物合成及HCV复制(8)中扮演重要角色。hnRNP R最近被鉴定，但它的功能仍然在研究中(9)，然而hnRNP R的生物学功能却与hnRNP Q不同。hnRNP Q和R蛋白出现在含有未翻译mRNAs的细胞质mRNP颗粒中(10)，且这两个蛋白都和SMN相互作用(11)。

1. Dreyfuss, G. et al. (1993) Annu Rev Biochem 62, 289-321.
2. Burd, C.G. and Dreyfuss, G. (1994) Science 265, 615-21.
3. Krecic, A.M. and Swanson, M.S. (1999) Curr Opin Cell Biol 11, 363-71.
4. Passos, D.O. et al. (2006) Biochem Biophys Res Commun 346, 517-25.
5. Moraes, K.C. et al. (2003) Biol Chem 384, 25-37.
6. Mourelatos, Z. et al. (2001) EMBO J 20, 5443-52.
7. Rossoll, W. et al. (2002) Hum Mol Genet 11, 93-105.
8. Liu, H.M. et al. (2009) Virology 386, 249-56.
9. Hassfeld, W. et al. (1998) Nucleic Acids Res 26, 439-45.
10. Carty, S.M. and Greenleaf, A.L. (2002) Mol Cell Proteomics 1, 598-610.

原厂资料：

Specificity / Sensitivity

hnRNP Q/R Antibody detects endogenous levels of hnRNP Q and hnRNP R proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human hnRNP Q. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Heterogeneous nuclear ribonucleoprotein Q and R belong to a family of hnRNP proteins that are involved in RNA binding, RNA biosynthesis, and mRNA transport from the nucleus to the cytoplasm (1-3). These two proteins are encoded by different genes but have 83% homology. hnRNP Q has three alternative splice variants (hnRNP Q1-3) (1-3). Methylation of carboxy-terminal arginine residues is required for nuclear localization (4). hnRNP Q binds to AU-rich mRNA in conjunction with AUF1 and regulates mRNA decay (5). hnRNP Q isoforms play a crucial role in mediating nuclear function of survival of motor neuron (SMN) complex (6,7) and modulating RNA biosynthesis and hepatitis C virus replication (8). hnRNP R was identified recently and its function is still under investigation (9), however hnRNP R does not duplicate the biological function of hnRNP Q. Both hnRNP Q and R are present in cytoplasmic mRNP granules containing untranslated mRNAs (10) and both interact with SMN (11).

1. Dreyfuss, G. et al. (1993) Annu Rev Biochem 62, 289-321.
2. Burd, C.G. and Dreyfuss, G. (1994) Science 265, 615-21.
3. Krecic, A.M. and Swanson, M.S. (1999) Curr Opin Cell Biol 11, 363-71.
4. Passos, D.O. et al. (2006) Biochem Biophys Res Commun 346, 517-25.
5. Moraes, K.C. et al. (2003) Biol Chem 384, 25-37.
6. Mourelatos, Z. et al. (2001) EMBO J 20, 5443-52.
7. Rossoll, W. et al. (2002) Hum Mol Genet 11, 93-105.
8. Liu, H.M. et al. (2009) Virology 386, 249-56.
9. Hassfeld, W. et al. (1998) Nucleic Acids Res 26, 439-45.
10. Carty, S.M. and Greenleaf, A.L. (2002) Mol Cell Proteomics 1, 598-610.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.