β-Tubulin (9F3) Rabbit mAb (HRP Conjugate)

• 产品编号：CST-5346S      品牌：CST       原厂货号：5346S
• 产品分类：抗体 > 一抗 > 酶标记一抗 > 辣根过氧化物酶（HRP）
• 应用分类：

描述：

β-Tubulin (9F3) Rabbit mAb (HRP Conjugate)兔单抗检测内源性β-tubulin总蛋白，但是不能与重组的α-tubulin蛋白发生交叉反应。通过人工合成人源β-tubulin蛋白氨基端相应的多肽片段去免疫动物从而制备出单克隆抗体。Cell Signaling Technology抗体通过它的氨基组连接着horseradish peroxidase (HRP)的碳水化合物组。HRP连接的抗体被期望与非连接的抗体(β-Tubulin (9F3) Rabbit mAb #2128)拥有相同的物种交叉反应活性。细胞骨架由三种类型的细胞质基质纤维组成：微管、微丝(肌动蛋白纤维)和中间纤维。球形微管蛋白亚基包含微管建筑块，由α/β-tubulin异源二聚体形成所有真核细胞的tubulin亚单位。γ-tubulin被需要在tubulin亚单位的成核聚合作用中去形成微管聚合物。许多细胞运动是通过微管动力介导的，包括纤毛和鞭毛的跳动、膜泡的核质转运、在减数分裂/有丝分裂期间染色体排布和神经轴突的迁移。这些运动由于竞争性微管聚合作用和解聚作用或通过微管动力蛋白的运动(1)。

1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Chicken

Specificity / Sensitivity

β-Tubulin (9F3) Rabbit mAb (HRP Conjugate) detects endogenous levels of total β-tubulin protein and does not cross-react with recombinant α-tubulin.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human β-tubulin.

Description

This Cell Signaling Technology (CST) antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody (β-Tubulin (9F3) Rabbit mAb #2128).

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).

1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA and 50%
glycerol. Store at -20ºC. Do not aliquot the antibody.