UVRAG Antibody

• 产品编号：CST-5320S      品牌：CST       原厂货号：5320S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

UVRAG Antibody 能够检测内源性的UVRAG总蛋白。该多克隆抗体通过合成的肽段免疫动物制备，这种合成肽的序列与人源UVRAG蛋白Leu555周围残基相一致。抗体通过蛋白A和多肽亲和色谱法制备。自噬是自噬溶酶体对其包裹的细胞质内容物进行降解的一种分解代谢过程(1,2)。自噬过程一般在营养匮乏的条件下被激活，但也与一些生理过程有关，如发育、分化、神经变性、感染和癌症(3)。关于自噬的分子机制，已在酵母中被大量发现，并且还提到了如自噬相关基因（Atg）。这些蛋白涉及称为自噬小体的细胞质液泡的形成，并且将其运送到溶酶体处降解。III型磷酸肌醇3-激酶(PI3KC3)/Vps34调节空泡的运输和自噬(4,5)。多种蛋白与Vsp34有关，包括：p105/Vsp15、Beclin-1、UVRAG、Atg14 和 Rubicon，这些蛋白能够决定Vsp34的功能(6-11)。UVRAG(紫外线辐射抗性相关基因)与Beclin-1/PI3KC3复合物相关，并提高PI3KC3酶活性，及促进自噬，抑制增殖(11)。Beclin-1结合UVRAG可以促进自噬小体的成熟和内吞小泡的运输(12)。同样地，UVRAG存在于肠癌和胃癌中，可以作为一个伴有移码突变的潜在的肿瘤抑制蛋白(13,14)。

1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
2. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18.
3. Corvera, S. (2001) Traffic 2, 859-66.
4. Stack, J.H. et al. (1995) J Cell Biol 129, 321-34.
5. Liang, C. et al. (2008) Nat Cell Biol 10, 776-87.
6. Matsunaga, K. et al. (2009) Nat Cell Biol 11, 385-96.
7. Zhong, Y. et al. (2009) Nat Cell Biol 11, 468-76.
8. Sun, Q. et al. (2008) Proc Natl Acad Sci U S A 105, 19211-6.
9. Itakura, E. et al. (2008) Mol Biol Cell 19, 5360-72.
10. Liang, C. et al. (2006) Nat Cell Biol 8, 688-99.
11. Ionov, Y. et al. (2004) Oncogene 23, 639-45.
12. Kim, M.S. et al. (2008) Hum Pathol 39, 1059-63.

原厂资料：

Specificity / Sensitivity

UVRAG Antibody detects endogenous levels of total UVRAG protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Leu555 of human UVRAG. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). It is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. These proteins are involved in the formation of cytoplasmic vacuoles called autophagasomes that are delivered to lysosomes for degradation. 
 The class III type phosphoinositide 3-kinase (PI3KC3)/Vps34 regulates vacuolar trafficking as well as autophagy (4,5). Multiple proteins have been shown to be associated with Vsp34, including: p105/Vsp15, Beclin-1, UVRAG, Atg14, and Rubicon, which can determine Vsp34 function (6-11). UVRAG (UV radiation resistance-associated gene) is associated with the Beclin-1/PI3KC3 complex and promotes PI3KC3 enzymatic activity and autophagy, while suppressing proliferation (11). Beclin-1 binding to UVRAG promotes both autophagosome maturation and endocytic trafficking (12). UVRAG is also a potential tumor suppressor protein with frameshift mutations observed in colon and gastric carcinomas (13,14).

1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
2. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18.
3. Corvera, S. (2001) Traffic 2, 859-66.
4. Stack, J.H. et al. (1995) J Cell Biol 129, 321-34.
5. Liang, C. et al. (2008) Nat Cell Biol 10, 776-87.
6. Matsunaga, K. et al. (2009) Nat Cell Biol 11, 385-96.
7. Zhong, Y. et al. (2009) Nat Cell Biol 11, 468-76.
8. Sun, Q. et al. (2008) Proc Natl Acad Sci U S A 105, 19211-6.
9. Itakura, E. et al. (2008) Mol Biol Cell 19, 5360-72.
10. Liang, C. et al. (2006) Nat Cell Biol 8, 688-99.
11. Ionov, Y. et al. (2004) Oncogene 23, 639-45.
12. Kim, M.S. et al. (2008) Hum Pathol 39, 1059-63.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.