SNAP25 (D7B4) Rabbit mAb

• 产品编号：CST-5308S      品牌：CST       原厂货号：5308S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SNAP25 (D7B4) Rabbit mAb 兔单抗识别内源性的SNAP25总蛋白。该单克隆抗体由合成的人类SNAP25蛋白Asp110位点附近肽段免疫动物而制成。25kDa的突触相关蛋白（SNAP25）是一个膜可溶性的、N-乙基马来酰亚胺敏感因子，可附着蛋白受体（T-SNARE），在神经元突触前膜被发现。SNAP25与SNARE蛋白syntaxin和synaptobrevin形成一个核心复合物，介导Ca2+依赖性胞吐时突触囊泡与质膜的融合（1）。这种复合物与神经递质γ-氨基丁酸（GABA）的胞外分泌的很有关。神经递质的释放可以被肉毒杆菌毒素A和E蛋白裂解SNAP25所抑制（2）。SNAP25在内体融合中和Q-SNARE类似起着次要的作用；该蛋白与注意缺陷多动障碍（ADHD）的遗传易感性相关（3）。

1. Salaün, C. et al. (2004) Biochim Biophys Acta 1693, 81-9.
2. Fan, H.P. et al. (2006) J Biol Chem 281, 28174-84.
3. Aikawa, Y. et al. (2006) Mol Biol Cell 17, 2113-24.

原厂资料：

Specificity / Sensitivity

SNAP25 (D7B4) Rabbit mAb detects endogenous levels of total SNAP25 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp110 of human SNAP25 protein.

Background

The 25 kDa synaptosome-associated protein (SNAP25) is a target membrane soluble, N-ethylmaleimide-sensitive factor attachment protein receptor (t-SNARE) that is found on neuronal presynaptic membranes. SNAP25 forms a core complex with the SNARE proteins syntaxin and synaptobrevin to mediate synaptic vesicle fusion with the plasma membrane during Ca2+-dependent exocytosis (1). This complex is responsible for exocytosis of the neurotransmitter γ-aminobutyric acid (GABA). Neurotransmitter release is inhibited by proteolysis of SNAP25 by botulinum toxins A and E (2). SNAP25 plays a secondary role as a Q-SNARE involved in endosome fusion; the protein is associated with genetic susceptibility to attention-deficit hyperactivity disorder (ADHD) (3).

1. Salaün, C. et al. (2004) Biochim Biophys Acta 1693, 81-9.
2. Fan, H.P. et al. (2006) J Biol Chem 281, 28174-84.
3. Aikawa, Y. et al. (2006) Mol Biol Cell 17, 2113-24.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.