GAP43 Antibody

• 产品编号：CST-5307S      品牌：CST       原厂货号：5307S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GAP43 Antibody 兔多抗识别内源性的总GAP蛋白。该多克隆抗体由合成的人类 GAP43蛋白氨基末端附近肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。GAP43是神经系统特异性生长相关蛋白，在生长锥和高可塑性区域含量丰富（1）。PKC磷酸化GAP43的Ser41，它由细胞内Ca2 +所调节，影响GAP43与钙调蛋白的结合能力（2,3）。GAP43与生长锥形成，神经轴突生长和大脑皮质功能的发展是不可分割的（4,5）。确诊为精神分裂症和阿尔茨海默氏病的患者可以有异常的GAP43表达（6,7）。

1. Biewenga, J.E. et al. (1996) Acta Biochim Pol 43, 327-38.
2. Dekker, L.V. and Parker, P.J. (1997) J Biol Chem 272, 12747-53.
3. Chapman, E.R. et al. (1991) J Biol Chem 266, 207-13.
4. Aigner, L. and Caroni, P. (1993) J Cell Biol 123, 417-29.
5. Shen, Y. et al. (2008) Cerebellum 7, 451-66.
6. Sower, A.C. et al. (1995) Mol Chem Neuropathol 24, 1-11.
7. de la Monte, S.M. et al. (1995) Am J Pathol 147, 934-46.

原厂资料：

Specificity / Sensitivity

GAP43 Antibody detects endogenous levels of total GAP protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GAP43 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

GAP43 is a nervous system specific, growth-associated protein enriched in growth cones and areas of high plasticity (1). Phosphorylation of GAP43 at Ser41 by PKC is regulated by intracellular Ca2+ and affects the ability of GAP43 to bind calmodulin (2,3). GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex (4,5). Aberrant GAP43 expression can be seen in patients diagnosed with schizophrenia and Alzheimer's disease (6,7).

1. Biewenga, J.E. et al. (1996) Acta Biochim Pol 43, 327-38.
2. Dekker, L.V. and Parker, P.J. (1997) J Biol Chem 272, 12747-53.
3. Chapman, E.R. et al. (1991) J Biol Chem 266, 207-13.
4. Aigner, L. and Caroni, P. (1993) J Cell Biol 123, 417-29.
5. Shen, Y. et al. (2008) Cerebellum 7, 451-66.
6. Sower, A.C. et al. (1995) Mol Chem Neuropathol 24, 1-11.
7. de la Monte, S.M. et al. (1995) Am J Pathol 147, 934-46.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.