ADH1 Antibody

• 产品编号：CST-5295S      品牌：CST       原厂货号：5295S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ADH1抗体用于检测内源性ADH1总蛋白水平。该抗原在人ADH1A, ADH1B 和 ADH1C中100%保守。多克隆抗体是通过合成对应于人ADH1B蛋白 Val74位点肽段免疫动物获得，抗体经Protein A和肽亲和层析纯化。人酒精脱氢酶基因（ADH）分为五大类，第一大类又包括三个不同基因ADH1A, ADH1B和ADH1C。ADH4, ADH5, ADH7 和ADH6 分属于第II，III，IV和V类。ADH是一种含锌的二聚体，在肝脏中与辅酶NAD一起将细胞内的酒精转化为乙醛（1）。ADH1A是单型性的，是胎儿和新生肝脏中的主要ADH酶。相反，多态性的ADH1B和ADH1C是成人肝脏中的主要形式（2）。人类1类ADH的多态性会导致功能性ADH酶的变异，这提示特定的基因变异可能跟酗酒有关（3）。

1. Edenberg, H.J. (2000) Prog Nucleic Acid Res Mol Biol 64, 295-341.
2. Su, J.S. et al. (2006) J Biol Chem 281, 19809-21.
3. Chen, C.C. et al. (1999) Am J Hum Genet 65, 795-807.

原厂资料：

Specificity / Sensitivity

ADH1 Antibody detects endogenous levels of total ADH1 protein. The antigen is 100% conserved between human ADH1A, ADH1B and ADH1C proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val74 of human ADH1B protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Human alcohol dehydrogenase (ADH) genes are grouped into five classes, with three distinct class I ADH genes (ADH1A, ADH1B and ADH1C) and ADH4, ADH5, ADH7 and ADH6 belonging to classes II, III, IV, and V, respectively. ADH is a zinc-containing, dimeric enzyme that catalyzes the conversion of cytosolic alcohol to acetaldehyde in the liver with the coenzyme NAD (1). ADH1A is monomorphic and is the predominant fetal and neonatal liver ADH enzyme. In contrast, polymorphic ADH1B and ADH1C enzymes are predominant in adult livers (2). Polymorphisms in the human class I ADH genes result in functionally variable ADH enzymes; evidence suggests that specific variants may provide protection from the risk of alcoholism (3).

1. Edenberg, H.J. (2000) Prog Nucleic Acid Res Mol Biol 64, 295-341.
2. Su, J.S. et al. (2006) J Biol Chem 281, 19809-21.
3. Chen, C.C. et al. (1999) Am J Hum Genet 65, 795-807.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody