BrdU (Bu20a) Mouse mAb

• 产品编号：CST-5292S      品牌：CST       原厂货号：5292S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

BrdU (Bu20a) Mouse mAb 能够检测掺入到单链DNA中的Brdu。DNA必须变性以暴露抗原表位供抗体检测。该单克隆抗体是由BSA偶联Brdu免疫动物生产的。卤代核苷酸如嘧啶类似物溴脱氧尿苷(BrdU)对于新生活细胞和组织中的DNA进行标记是有用的。BrdU掺入复制的DNA后代替胸苷，随后使用允许细胞周期中S期标记的细胞特异性单克隆抗体对BrdU进行免疫检测。溴脱氧尿苷脉冲标记细胞或组织后，BrdU (Bu20a)小鼠单克隆抗体可用于检测掺入单链DNA的BrdU。请参阅我们有关标记程序的详细步骤，以及应用于各种免疫检测的双链DNA变性程序(1-4)。

1. Darzynkiewicz, Z. and Juan, G. (2001) Curr Protoc Cytom Chapter 7, Unit 7.7.
2. Leif, R.C. et al. (2004) Cytometry A 58, 45-52.
3. Staszkiewicz, J. et al. (2009) Biochem Biophys Res Commun 378, 539-44.
4. Rothaeusler, K. and Baumgarth, N. (2007) Curr Protoc Cytom Chapter 7, Unit7.31.

原厂资料：

Specificity / Sensitivity

BrdU (Bu20a) Mouse mAb detects BrdU when incorporated into single stranded DNA. DNA must be denatured for the epitope to be exposed and recognized by the antibody.

Source / Purification

Monoclonal antibody is produced by immunizing animals with BrdU conjugated to BSA.

Background

Halogenated nucleotides such as the pyrimidine analog bromodeoxyuridine (BrdU) are useful for labeling nascent DNA in living cells and tissues. BrdU becomes incorporated into replicating DNA in place of thymidine and subsequent immunodetection of BrdU using specific monoclonal antibodies allows labeling of cells in S phase of the cell cycle. After pulse-labeling cells or tissues with bromodeoxyuridine, BrdU (Bu20a) Mouse mAb can be used to detect BrdU incorporated into single stranded DNA. Please see our detailed protocol for information regarding the labeling procedure as well as denaturation of double stranded DNA for various immunodetection applications (1-4).

1. Darzynkiewicz, Z. and Juan, G. (2001) Curr Protoc Cytom Chapter 7, Unit 7.7.
2. Leif, R.C. et al. (2004) Cytometry A 58, 45-52.
3. Staszkiewicz, J. et al. (2009) Biochem Biophys Res Commun 378, 539-44.
4. Rothaeusler, K. and Baumgarth, N. (2007) Curr Protoc Cytom Chapter 7, Unit7.31.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.