Gα(i) Antibody

• 产品编号：CST-5290S      品牌：CST       原厂货号：5290S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Gα(i)抗体识别内源性的全部Gα(i)蛋白。该多克隆抗体用与人类Gα(i)中Arg100位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。异三聚鸟嘌呤核苷酸结合蛋白（G蛋白）由α、β和γ亚基构成，介导激素、神经递质、趋化因子和感觉刺激的作用。到今天为止，根据结构和功能的相似性，超过20个亚基分为四个家族，分别是Gα(s), Gα(i/o), Gα(q)和Gα(12)（1,2）。Gα(q)/Gα(11)的Tyr356磷酸化对G蛋白激活是至关重要的，因为苯丙氨酸替代Tyr356改变了Gα与受体的相互作用，并消除了配体诱导的IP3的形成（3）。Gα(i)可导致腺苷酸环化酶的抑制，导致细胞内cAMP水平下降。百日咳毒素催化Gα(i)的ADP-核糖基化，使得Gα(i)失活，减轻其对腺苷酸环化酶的抑制（4）。

1. Offermanns, S. (2001) Oncogene 20, 1635-42.
2. Pierce, K.L. et al. (2002) Nat Rev Mol Cell Biol 3, 639-50.
3. Umemori, H. et al. (1997) Science 276, 1878-81.
4. Tsai, S.C. et al. (1984) J Biol Chem 259, 15320-3.

原厂资料：

Specificity / Sensitivity

Gα(i) Antibody detects endogenous levels of total Gα(i) protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg100 of human Gα(i). Antibodies are purified by protein A and peptide affinity chromatography.

Background

Heterotrimeric guanine nucleotide-binding proteins (G proteins) consist of α, β and γ subunits and mediate the effects of hormones, neurotransmitters, chemokines and sensory stimuli. To date, over 20 known Gα subunits have been classified into four families, Gα(s), Gα(i/o), Gα(q) and Gα(12), based on structural and functional similarities (1,2). Phosphorylation of Tyr356 of Gα(q)/Gα(11) is essential for activation of the G protein, since phenylalanine substitution for Tyr356 changes the interaction of Gα with receptors and abolishes ligand-induced IP3 formation (3).Gα(i) causes inhibition of adenylate cyclase, leading to a decrease in cellular levels of cAMP. Pertussis toxin catalyzes ADP-ribosylation of Gα(i), which inactivates the Gα(i) protein and attenuates inhibition of adenylate cyclase (4).

1. Offermanns, S. (2001) Oncogene 20, 1635-42.
2. Pierce, K.L. et al. (2002) Nat Rev Mol Cell Biol 3, 639-50.
3. Umemori, H. et al. (1997) Science 276, 1878-81.
4. Tsai, S.C. et al. (1984) J Biol Chem 259, 15320-3.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.