HMGA2 Antibody

• 产品编号：CST-5269S      品牌：CST       原厂货号：5269S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

HMGA2 Antibody能够检测内源性HMGA2总蛋白水平。通过合成的与人源HMGA2蛋白氨基端相应的多肽片段去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。HMGA2蛋白属于拥有AT-hook DNA结合域的高速泳动族蛋白high mobility group protein（HMG）家族。HMGA蛋白被认为是结构转录因子（architectural transcription factor）；它们没有直接的转录激活能力，但是通过改变的DNA构象结合到DNA的AT富含区域从而代替调节基因表达和/huo直接与其它转录因子发生相互作用(1,2)。HMGA2蛋白大量地和广泛的表达，并且在胚胎发育期间起着关键作用(3)。HMGA2蛋白促进干细胞自我更新，以及它的表达减少是与干细胞衰老有关(4-7)。在正常成年组织中HMGA2蛋白的表达水平是非常低，但是它的过表达或重新布置使与许多癌症形式有关的(8-11)。

1. Cleynen, I. and Van de Ven, W.J. (2008) Int J Oncol 32, 289-305.
2. Pfannkuche, K. et al. (2009) Stem Cell Rev 5, 224-30.
3. Monzen, K. et al. (2008) Nat Cell Biol 10, 567-74.
4. Nishino, J. et al. (2008) Cell 135, 227-39.
5. Li, O. et al. (2006) Genesis 44, 523-9.
6. Li, O. et al. (2007) FEBS Lett 581, 3533-7.
7. Fusco, A. and Fedele, M. (2007) Nat Rev Cancer 7, 899-910.
8. Rawlinson, N.J. et al. (2008) Cancer Genet Cytogenet 181, 119-24.
9. Wei, J.J. et al. (2010) Am J Surg Pathol 34, 18-26.
10. Mahajan, A. et al. (2010) Mod Pathol 23, 673-81.

原厂资料：

Specificity / Sensitivity

HMGA2 Antibody detects endogenous levels of total HMGA2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminus of human HMGA2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

HMGA2 belongs to the family of high mobility group with AT-hook DNA binding domain. HMGA proteins are considered architectural transcription factors; they do not have direct transcriptional activation capacity, but instead regulate gene expression by changing DNA conformation through binding to AT-rich regions in the DNA, and/or direct interaction with other transcription factors (1,2). HMGA2 is abundantly and ubiquitously expressed and plays a crucial role during embryonic development (3). HMGA2 promotes stem cell self-renewal and decreases in its expression are associated with stem cell aging (4-7). Expression levels of HMGA2 are very low in normal adult tissues, but its overexpression or rearrangement is associated with many types of cancer (8-11).

1. Cleynen, I. and Van de Ven, W.J. (2008) Int J Oncol 32, 289-305.
2. Pfannkuche, K. et al. (2009) Stem Cell Rev 5, 224-30.
3. Monzen, K. et al. (2008) Nat Cell Biol 10, 567-74.
4. Nishino, J. et al. (2008) Cell 135, 227-39.
5. Li, O. et al. (2006) Genesis 44, 523-9.
6. Li, O. et al. (2007) FEBS Lett 581, 3533-7.
7. Fusco, A. and Fedele, M. (2007) Nat Rev Cancer 7, 899-910.
8. Rawlinson, N.J. et al. (2008) Cancer Genet Cytogenet 181, 119-24.
9. Wei, J.J. et al. (2010) Am J Surg Pathol 34, 18-26.
10. Mahajan, A. et al. (2010) Mod Pathol 23, 673-81.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody