Oct-4A (C30A3) Rabbit mAb (Alexa Fluor? 647 Conjugate)

• 产品编号：CST-5263S      品牌：CST       原厂货号：5263S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Oct-4A (C30A3)Rabbit mAb兔单抗 (Alexa Fluor^® 647 偶联)可以检测内源性的总Oct-4A蛋白。合成与人Oct-4A蛋白氨基末端的氨基酸序列一致的重组蛋白，免疫动物获得单克隆抗体。Cell Signaling Technology公司抗体与Alexa Fluor^® 647 荧光染料偶联，经直接流式细胞术和免疫荧光分析对人细胞进行内部测试。本抗体与非偶联的Oct-4A (C30A3) Rabbit mAb兔单抗 #2840 有同样的种属交叉反应。Oct-4 (POU5F1)是一类高表达于未分化胚胎干细胞和原始胚胎生殖细胞中的转录因子（1）,一系列关键因子包括Oct-4, Nanog和 Sox2对保持多潜能性是必需的，Oct-4的下调显示能起始细胞分化（2,3）。Oct-4是一种非常有用的生殖细胞肿瘤标志物[4]。Oct-4存在两种剪接异构体Oct-4A and Oct-4B（5）。近来研究暗示Oct-4A异构体能给予并保持干细胞多潜能，而Oct-4B可能存在于某些体细胞和非多潜能性细胞中（6,7）。

1. Looijenga, L.H. et al. (2003) Cancer Res 63, 2244-50.
2. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-278.
3. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
4. Cheng, L. et al. (2007) J Pathol 211, 1-9.
5. Takeda, J. et al. (1992) Nucleic Acids Res 20, 4613-20.
6. Cauffman, G. et al. (2006) Stem Cells 24, 2685-91.
7. Lee, J. et al. (2006) J Biol Chem 281, 33554-65.

原厂资料：

Specificity / Sensitivity

Oct-4A (C30A3) Rabbit mAb (Alexa Fluor^® 647 Conjugate) detects endogenous levels of total Oct-4A protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Oct-4A protein.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor^® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Oct-4A (C30A3) Rabbit mAb #2840.

Background

Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that it is the Oct-4A isoform that has the ability to confer and sustain pluripotency and that Oct-4B may exist in some somatic, non-pluripotent cells (6,7).

1. Looijenga, L.H. et al. (2003) Cancer Res 63, 2244-50.
2. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-278.
3. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
4. Cheng, L. et al. (2007) J Pathol 211, 1-9.
5. Takeda, J. et al. (1992) Nucleic Acids Res 20, 4613-20.
6. Cauffman, G. et al. (2006) Stem Cells 24, 2685-91.
7. Lee, J. et al. (2006) J Biol Chem 281, 33554-65.

注意事项：

Storage: Supplied in PBS (pH 7.2), 2 mg/ml BSA and less than
0.1% sodium azide. Store at 4°C. Protect from light.
Do not freeze.