COX IV (3E11) Rabbit mAb (HRP Conjugate)

• 产品编号：CST-5247S      品牌：CST       原厂货号：5247S
• 产品分类：抗体 > 一抗 > 酶标记一抗 > 辣根过氧化物酶（HRP）
• 应用分类：

描述：

COX IV (3E11) Rabbit mAb 兔单抗(HRP Conjugate)用于检测内源性COX IV总蛋白水平。该单抗通过用对应于人源COX IV蛋白Lys29位点周围残基序列的合成肽免疫动物制备而得。该抗体通过其氨基与辣根过氧化物酶（HRP）的碳水化合物基团共价连接。酶连抗体的交互反应性应等同于非交连的抗体（COX IV (3E11) Rabbit mAb 兔单抗#4850）。细胞色素C氧化酶(COX)是由13个亚基组成的定位于线粒体内膜的异寡聚体（1-3）。它是线粒体氧化呼吸链的最后一个环节的酶复合体，负责将氧分子还原为水，并且将该反应与质子跨线粒体内膜并驱动ATP的合成反应相耦联。该复合体催化核心的三个大的亚基是由线粒体DNA编码的，而其它的小的亚基，包括COX IV是核基因编码。COX基因的缺陷与很多人类疾病有关（4）。COX IV抗体经常作为线粒体内参对照使用。

1. Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466.
2. Capaldi, R.A. et al. (1983) Biochim. Biophys. Acta 726, 135-148.
3. Kadenbach, B. et al. (2000) Free Radic. Biol. Med. 29, 211-221.
4. Barrientos, A. et al. (2002) Gene 286, 53-63.

原厂资料：

Specificity / Sensitivity

COX IV (3E11) Rabbit mAb (HRP Conjugate) detects endogenous levels of total COX IV protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys29 of human COX IV.

Description

This Cell Signaling Technology (CST) antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody (COX IV (3E11) Rabbit mAb #4850).

Background

Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA while the other smaller subunits, including COX IV, are nuclear-encoded. Deficiency in COX activity has been correlated with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control.

1. Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466.
2. Capaldi, R.A. et al. (1983) Biochim. Biophys. Acta 726, 135-148.
3. Kadenbach, B. et al. (2000) Free Radic. Biol. Med. 29, 211-221.
4. Barrientos, A. et al. (2002) Gene 286, 53-63.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA and 50%
glycerol. Store at -20ºC. Do not aliquot the antibody.