Human Insulin-like Growth Factor II (hIGF-II)

• 产品编号：CST-5238SC      品牌：CST       原厂货号：5238SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > IGF 胰岛素样生长因子及其结合蛋白
• 应用分类：

描述：

重组的人IGF-II (hIGF-II) Ala25-Glu91（产品号#NP_000603）由CST在大肠杆菌中生产。重组的hIGF-II在氨基端没有甲硫氨酸，分子量为7,606。DTT还原的和未还原的重组蛋白电泳条带对应于6 kDa多肽。重组蛋白氨基端的预测AYRPS由氨基酸测序进行验证。经SDS-PAGE变性胶和非变性胶确定，重组蛋白纯度大于98%。重组的hIGF-II生物活性用人原代皮肤成纤维细胞的细胞增殖实验进行确定。每个lot的半数有效量为10－20 ng/ml之间。胰岛素样生长因子II（IGF-II）是与胰岛素样生长因子1（IGF－I）紧密相关的一个潜在的细胞有丝分裂原（1）。IGF-II主要在肝脏中产生，在多种肿瘤细胞中高表达（1,2）。IGF-II与其受体结合后，会激活AKT, mTOR, ERK和JNK通路（1）。IGF-II信号由多种不同的机制调控。首先，IGF结合蛋白会与IGF-II结合，并阻断其与受体的结合（1－3）。其次，IGF-IIR可以作为IGF-II的分子阱与其结合（1－3）。最后，IGF2 是一个印迹基因，印迹的缺失会引起IGF2 水平的上升（1－3）。IGF2 的异常表达跟肾的母细胞瘤，Beckwith-Wiedmann综合症和结直肠癌有关（1,2）。

原厂资料：

Source / Purification

Recombinant human IGF-II (hIGF-II) Ala25-Glu91 (Accession #NP_000603) was produced in E. coli at Cell Signaling Technology.

Molecular Characterization

Recombinant hIGF-II does not have a Met on the amino terminus and has a calculated MW of 7,606. DTT-reduced and non-reduced protein migrate as 6 kDa polypeptides. The expected amino-terminal AYRPS of recombinant hIGF-II was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIGF-II. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hIGF-II was determined in a cell proliferation assay using primary human dermal fibroblasts. The ED50 of each lot is between 10-20 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hIGF-II.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg hIGF-II. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl.

Background

IGF-II is a potent cellular mitogen that is closely related to IGF-I (1). IGF-II is primarily produced by the liver and is frequently overexpressed in tumors (1,2). IGF-II binds to the IGF-IR, activating the AKT, mTOR, ERK, and JNK pathways (1). IGF-II signaling is regulated by several distinct mechanisms. First, IGF binding proteins (IGFBPs) bind to IGF-II and block interactions with the IGF-IR (1-3). Second, the IGF-IIR, binds to and acts as a molecular trap for IGF-II (1-3). Lastly, the IGF2 gene is an imprinted gene, and loss of imprinting leads to increased IGF-II levels (1-3). Aberrant levels of IGF-II are associated with Wilms tumor, Beckwith-Wiedmann syndrome, and colorectal cancer (1,2).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.