Mouse Interleukin-6 (mIL-6)

• 产品编号：CST-5216SC      品牌：CST       原厂货号：5216SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > IL-6 Family 白细胞介素-6超家族
• 应用分类：

描述：

CST公司利用重组的鼠IL-6 (mIL-6) Phe25-Thr211 (Accession #NP_112445)在人的293细胞中表达。重组的mIL-6蛋白在末端没有标签，未糖基化的蛋白分子量据推算为21,734Da。DTT-还原和未还原的蛋白迁移时是作为一个31kDa 的多肽而转移。其在SDS-PAGE胶中的低迁移性质是由于糖基化。重组mIL-6蛋白的N-末端序列FPTSQ通过测序得到。6 μg 还原 (+) 和 非还原的(-) mIL-6通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组蛋白mIL-6的生物活性是通过B9细胞增殖实验确定的。每个批次的ED50在0.5-2pg/ml之间。

有载体: 每微克mIL-6蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克mIL-6蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

IL-6是一个潜在的急性期应答的诱导因子，它由T细胞、巨噬细胞、纤维原细胞、内皮细胞和其它细胞产生(1,2)。IL-6诱导细胞增殖和分化并作用于B细胞、T细胞、胸腺细胞和其它细胞之中。IL-6与TGF-β相作用对Th17应答的发展非常重要。IL-6与IL-6Rα结合并通过此结合诱导gp130的同型二聚化(1)。gp130的同型二聚化引起Jak/Stat级联反应和SHP2/MAPK (Erk)级联反应(1,3,4)。IL-6也能与IL-6Rα可变剪切形成与膜无关的复合体(3)。IL-6/可溶性 IL-6Rα 复合体在表达gp130的细胞上能激活gp130信号通路，但不是IL-6Rα (3)。一些研究证明IL-6可能通过增加促血管新生因子 VEGF对乳腺癌的转移有作用(5)。

原厂资料：

Source / Purification

Recombinant mouse IL-6 (mIL-6) Phe25-Thr211 (Accession #NP_112445) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant mIL-6 contains no "tags" and the nonglycosylated protein has a calculated MW of 21,734. DTT-reduced and non-reduced protein migrate as 31 kDa polypeptides. Lower mobility in SDS-PAGE is due to glycosylation. The expected amino-terminal FPTSQ of recombinant mIL-6 was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-6. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant mIL-6 was determined in a B9 cell proliferation assay. The ED50 of each lot is between 0.5 and 2 pg/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg mIL-6.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-6. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

IL-6 is a potent inducer of the acute phase response and is produced by T cells, macrophages, fibroblasts, endothelial, and other cells (1,2). IL-6 induces proliferation and differentiation and acts on B cells, T cells, thymocytes, and others. IL-6 in concert with TGFβ is important for developing Th17 responses. IL-6 binds to IL-6Rα and through this association induces gp130 homodimerization (1). gp130 homodimerization triggers the Jak/Stat cascade and the SHP2/MAPK (Erk) cascade (1,3,4). IL-6 also forms a complex with an IL-6Rα splice variant that is non-membrane associated (3).The IL-6/soluble IL-6Rα complex can then activate the gp130 signaling pathway on cells that express gp130 but not IL-6Rα (3). IL-6, through increasing expression of proangiogenic VEGF, may contribute to metastatic breast cancer (5).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles