PCM-1 (G2000) Antibody

• 产品编号：CST-5213S      品牌：CST       原厂货号：5213S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PCM-1 (G2000) Antibody识别内源性PCM-1蛋白。通过人工合成人源PCM-1蛋白羧基末端相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。中心体是由一对周围为云状电子致密物的中心粒组成，并且其功能是微管组织中心(Microtubule-Organizing Center,MTOC）负责微管成核和在细胞周期进程中纺锤体的组织(1)。Percentriolar material 1 (PCM-1)蛋白是一个228 kDa大分子蛋白，其以细胞周期依赖的方式与中心体相关(2) 。PCM-1蛋白位于小的细胞质颗粒又中心体的卫星(3)。PCM-1蛋白对于数种中心体蛋白包括centrin、pericentrin、ninein、NEK2和CEP250的装配是需要的(4-8)。染色体易位涉及编码PCM-1蛋白和酪氨酸激酶Ret蛋白的基因，以及Jak2蛋白与一些癌症包括乳头状甲状腺癌和粒细胞性白血病有关联(9-11)。

1. Zimmerman, W. and Doxsey, S.J. (2000) Traffic 1, 927-34.
2. Balczon, R. et al. (1994) J Cell Biol 124, 783-93.
3. Kubo, A. and Tsukita, S. (2003) J Cell Sci 116, 919-28.
4. Kim, J. et al. (2008) Hum Mol Genet 17, 3796-805.
5. Dammermann, A. and Merdes, A. (2002) J Cell Biol 159, 255-66.
6. Li, Q. et al. (2001) J Cell Sci 114, 797-809.
7. Hames, R.S. et al. (2005) Mol Biol Cell 16, 1711-24.
8. Mack, G.J. et al. (1998) Arthritis Rheum 41, 551-8.
9. Corvi, R. et al. (2000) Oncogene 19, 4236-42.
10. Reiter, A. et al. (2005) Cancer Res 65, 2662-7.
11. Bousquet, M. et al. (2005) Oncogene 24, 7248-52.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

PCM-1 (G2000) Antibody detects endogenous levels of PCM-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region near the carboxyl terminus of human PCM-1 protein. Antibodies were purified by protein A and peptide affinity chromatography.

Background

The centrosome is composed of a pair of centrioles surrounded by electron-dense pericentriolar material and functions as the microtubule-organizing center responsible for microtubule nucleation and spindle organization during cell cycle progression (1). Percentriolar material 1 (PCM-1) is a large, 228 kDa protein associated with the centrosome in a cell cycle dependent manner (2). PCM-1 localizes to small cytoplasmic granules called centrosomal satellites (3). PCM-1 is required for the assembly of several centrosomal proteins including centrin, pericentrin, ninein, NEK2, and CEP250 (4-8). Chromosomal translocations involving genes encoding PCM-1 and the tyrosine kinases Ret and Jak2 are associated with some cancers, including papillary thyroid carcinoma and myeloid leukemia (9-11).

1. Zimmerman, W. and Doxsey, S.J. (2000) Traffic 1, 927-34.
2. Balczon, R. et al. (1994) J Cell Biol 124, 783-93.
3. Kubo, A. and Tsukita, S. (2003) J Cell Sci 116, 919-28.
4. Kim, J. et al. (2008) Hum Mol Genet 17, 3796-805.
5. Dammermann, A. and Merdes, A. (2002) J Cell Biol 159, 255-66.
6. Li, Q. et al. (2001) J Cell Sci 114, 797-809.
7. Hames, R.S. et al. (2005) Mol Biol Cell 16, 1711-24.
8. Mack, G.J. et al. (1998) Arthritis Rheum 41, 551-8.
9. Corvi, R. et al. (2000) Oncogene 19, 4236-42.
10. Reiter, A. et al. (2005) Cancer Res 65, 2662-7.
11. Bousquet, M. et al. (2005) Oncogene 24, 7248-52.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.