Mouse Vascular Endothelial Growth Factor-164 (mVEGF₁₆₄ )

• 产品编号：CST-5211LF      品牌：CST       原厂货号：5211LF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > VEGF血管内皮生长因子
• 应用分类：

描述：

CST公司生产的鼠源重组蛋白VEGF164 (mVEGF164) Ala205-Arg368 (Accession #NP_033531)是从人源293细胞表达而来。重组的mVEGF164蛋白没有标签，未糖基化的蛋白分子量据推算为19,278Da。DTT-还原蛋白由于糖基化的存在而作为一个24-31kDa的蛋白转移,因此在SDS-PAGE中有较低的迁移率。未还原的蛋白由于有半胱氨酸连接形成同源二聚体而作为38-44kDa 蛋白迁移。重组hVEGF164蛋白的N-末端APTTE的序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) mVEGF164通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组蛋白mVEGF164的生物活性是通过HUVEC细胞的增殖实验确定的。每个批次的ED50值在1-5 ng/ml之间。

有载体: 每微克mVEGF164蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克mVEGF164蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

VEGF164是鼠源VEGF-A基因众多剪切形式中的一种, 比其在人中的相似剪切形式VEGF165只短一个氨基酸(1,2)。VEGF164是通过很多细胞产生包括内皮细胞、巨噬细胞、T细胞(1,2)。VEGF165参与到了血管再生、血管内皮细胞生存、生长、迁移和血管渗透(1,2)。 基因的表达受到组织缺氧、炎症因子和肿瘤组织产生的致癌基因的诱导(1,2)。VEGF164结合到硫酸乙酰肝素并返回细胞表面和细胞间质中(1-3)。VEGF164的受体是VEGFR1和VEGFR2酪氨酸受体激酶(2)。NRP-1和NRP-2 可能作为共受体并增强VEGFR2信号(2-3)。VEGF164 结合到 VEGFR1和VEGFR2导致了信号通路的激活，如PI3K/AKT, P38 MAPK, FAK和桩蛋白(2)。

原厂资料：

Source / Purification

Recombinant mouse VEGF164 (mVEGF164) Ala205-Arg368 (Accession #NP_033531) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant mVEGF164 contains no "tags" and the nonglycosylated protein has a calculated MW of 19,278. DTT-reduced protein migrates as a 24-31 kDa polypeptide. Lower mobility in SDS-PAGE is due to glycosylation. The non-reduced cystine-linked homodimer migrates as a 38-44 kDa protein. The expected amino-terminal APTTE of recombinant mVEGF164 was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mVEGF164. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant mVEGF164 was determined in a cell proliferation assay using HUVEC. The ED50 of each lot is between 1-5 ng/ml.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mVEGF164. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

VEGF164 is one of many splice variants of the mouse VEGF-A gene, and is one amino acid shorter than its human counterpart, VEGF165 (1,2). VEGF164 is produced by a number of cells including endothelial cells, macrophages and T cells (1,2). VEGF164 is involved in angiogenesis, vascular endothelial cell survival, growth, migration and vascular permeability (1,2). Gene expression is induced by hypoxia, inflammatory cytokines and oncogenes (1,2). VEGF164 binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix (1-3). VEGFR1 and VEGFR2 are the receptor tyrosine kinases for VEGF164 (2). NRP-1 and NRP-2 may function as co-receptors and enhance VEGFR2 signaling (2-3). Binding of VEGF164 to VEGFR1 and VEGR2 leads to activation of the PI3K/AKT, p38 MAPK, FAK and Paxillin (2).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.