VEGF Receptor 2 (55B11) Rabbit mAb (Sepharose Bead^? Conjugate)

• 产品编号：CST-5168S      品牌：CST       原厂货号：5168S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

VEGF Receptor 2 (55B11) 兔单抗(琼脂糖珠偶联) 用于检测内源VEGF receptor 2 的蛋白表达水平。本抗体不与家族中其它成员发生交叉反应。单克隆抗体用含有人VEGF receptor 2 羧基端150个氨基酸的重组蛋白免疫动物得到。Cell Signaling Technology生产的抗体通过初级氨基基团与带有N-羟基琥珀酰亚胺的琼脂糖珠子共价结合。VEGF Receptor 2 (55B11)兔单抗 (琼脂糖珠偶联)可用于免疫沉淀检测。该抗体与未结合珠子的VEGF Receptor 2 (55B11) 兔单抗 #2479有着相同的种属交叉反应特性。血管内皮生长因子受体2 (VEGFR2, KDR, Flk-1)是内皮细胞上VEGF诱导信号时的一个主要受体。和配体结合之后，VEGFR2发生自磷酸化并被活化(1)。VEGFR2 主要的自磷酸化受体位于激酶插入区 (Tyr951/996)和酪氨酸激酶催化区(Tyr1054/1059) (2)。受体的激活会导致对包括Shc, Grb2, PI3K激酶, Nck 和蛋白酪氨酸磷酸化酶SHP-1 和 SHP-2接头蛋白的快速招募(3)。Tyr1212的磷酸化为Grb2提供了结合位点而且磷酸化的Tyr1175能结合PI3K激酶的p85 亚基以及PLCγ和 Shb (1,4,5)。VEGFR2信号传导对于实现VEGF刺激引起的增殖、趋化和发芽，以及体外培养的内皮细胞的增殖和体内血管的发生是必需的(6-8)。

1. Meyer, M. et al. (1999) EMBO J 18, 363-74.
2. Dougher-Vermazen, M. et al. (1994) Biochem Biophys Res Commun 205, 728-38.
3. Kroll, J. and Waltenberger, J. (1997) J Biol Chem 272, 32521-7.
4. Takahashi, T. et al. (2001) EMBO J 20, 2768-78.
5. Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75.
6. Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605.
7. Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92.
8. Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine

Specificity / Sensitivity

VEGF Receptor 2 (55B11) Rabbit mAb (Sepharose Bead Conjugate) detects endogenous levels of VEGF receptor 2 protein. This antibody does not cross-react with other family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein containing the carboxy-terminal 150 amino acid residues of human VEGF receptor 2 protein.

Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. VEGF Receptor 2 (55B11) Rabbit mAb (Sepharose Bead Conjugate) is useful for immunoprecipitation assays. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated VEGF Receptor 2 (55B11) Rabbit mAb #2479.

Background

Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, Grb2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for Grb2 binding and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).

1. Meyer, M. et al. (1999) EMBO J 18, 363-74.
2. Dougher-Vermazen, M. et al. (1994) Biochem Biophys Res Commun 205, 728-38.
3. Kroll, J. and Waltenberger, J. (1997) J Biol Chem 272, 32521-7.
4. Takahashi, T. et al. (2001) EMBO J 20, 2768-78.
5. Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75.
6. Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605.
7. Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92.
8. Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not
aliquot the antibody.