Loading Control Antibody Sampler Kit

产品编号：CST-5142S 品牌：Cell Signaling Technology 原厂货号：5142S
产品分类：抗体 > 一抗 > 复合抗体试剂盒
应用分类：

包装：

1 Kit

运保温度:

–20°C

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其它组分：

β-Tubulin (9F3) Rabbit mAb 【子货号：#2128，包装：40 µl，，运保温度：–20°C】

Histone H3 (D1H2) XP® Rabbit mAb 【子货号：#4499，包装：40 µl，，运保温度：–20°C】

COX IV (3E11) Rabbit mAb 【子货号：#4850，包装：40 µl，，运保温度：–20°C】

GAPDH (D16H11) XP® Rabbit mAb 【子货号：#5174，包装：40 µl，，运保温度：–20°C】

Anti-rabbit IgG, HRP-linked Antibody 【子货号：#7074，包装：100 µl，，运保温度：–20°C】

β-Actin (D6A8) Rabbit mAb 【子货号：#8457，包装：40 µl，，运保温度：–20°C】

描述：

单克隆抗体是通过采用分别与人类β-actin氨基末端、人类COX IV周围的Lys29、人类GAPDH序列、人类组蛋白H3羧基末端残基和人类β-actin氨基末端残基相对应的残基合成的肽段免疫动物生产的。上样对照小包装抗体套装为检测多种管家蛋白提供了经济的检测方法。本试剂盒包括足量的一抗和二抗来做四次western blots实验 /一抗。管家蛋白在细胞中执行多种功能，连续高表达在多种组织和细胞中。Western blot 方法一般采用管家蛋白如β-actin, COX IV , GAPDH ,组蛋白H3和 α-与β-tubulin作为上样对照。Actin是一种泛素化蛋白，是真核细胞骨架的重要组成成分，通常以F-actin纤维多聚体的形式存在（1）。甘油醛-3-磷酸脱氢酶（GAPDH）在糖生成过程中参与催化甘油醛-3-磷酸发生磷酸化。近期的研究表明GAPDH在细胞凋亡（2）基因表达（3）和核转运（4）中起着非常重要的作用。球形微球蛋白亚基由α- and β-tubulin组成，是微管蛋白的构成成分。而后者是构成细胞骨架的三种细胞质纤维蛋白之一（5）。组蛋白蛋白质包括组蛋白H3，组成染色质的初级结构，也称为核小体。染色质结构的变化对调节真核细胞的转录起着非常重要的作用（6）。细胞色素氧化酶（COX）是一种异寡聚酶，包含位于线粒体内膜上的13个亚基（7-9）。它是在呼吸链中的末端酶复合体，参与催化质子的还原，跨过线粒体内膜促进ATP的合成（10）。

原厂资料：

Specificity / Sensitivity

Each antibody in the Loading Control Antibody Sampler Kit detects endogenous levels of its target protein and does not typically cross-react with other proteins. β-Actin (13E5) Rabbit mAb detects endogenous levels of total β-actin protein and may cross-react with γ-actin.

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues near the amino terminus of human β-actin, surrounding Lys29 of human COX IV, the sequence of human GAPDH, the carboxy-terminal residues of human histone H3, and the amino terminus of human β-tubulin.

Description

The Loading Control Antibody Sampler Kit contains antibodies to a variety of housekeeping proteins. The kit contains enough primary and secondary antibodies to perform four western blots per primary antibody.

Background

Housekeeping proteins perform numerous basic functions within the cell and are constitutively expressed at high levels in a variety of tissues and cell types. Western blot analysis commonly uses housekeeping proteins such as β-actin, COX IV, GAPDH, histone H3 and the α- and β-tubulins as loading controls. Actin is a ubiquitous protein and a major component of the eukaryotic cytoskeleton. Actin exists mainly as the F-actin fibrous polymer (1). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Recent work has demonstrated that GAPDH plays roles in apoptosis (2), gene expression (3), and nuclear transport (4). Globular tubulin subunits made up of α- and β-tubulin heterodimers are the building blocks of microtubules, one of three types of cytosolic fibers that comprise the cytoskeleton (5). Histone proteins, including histone H3, make up the primary building block of chromatin known as nucleosomes. Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes (6). Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (7-9). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of protons across the mitochondrial inner membrane to drive ATP synthesis (10).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies