PhosphoPlus? LKB1 (Ser428) Antibody Duet

• 产品编号：CST-5132S      品牌：CST       原厂货号：5132S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

CST PhosphoPlus®抗体套装用于测定蛋白的活性状态，每个套装中包括目标蛋白总蛋白抗体和活化态蛋白抗体。CST抗体套装中的抗体都是从产品中质量上乘的抗体挑选出来的。LKB1 (STK11)是一种丝氨酸/苏氨酸蛋白激酶，通过调控多种下游激酶信号通路来调控细胞结构，凋亡和能量平衡（1,2）。一个包括有LKB1，假定的激酶STRAD和MO25 脚手架蛋白和胞质蛋白复合体可以激活AMPK和AMPK相关激酶的活性（3）。AMPK是细胞能量平衡中的主要调控蛋白，控制多种下游的效应蛋白，在接受ATP浓度信号时发挥细胞生长和凋亡的调控作用（4）。在细胞内LKB1可以在多个位点被磷酸化，包括 Ser31/325/428 和 Thr189/336/363 (5)。LKB1的突变会引起Peutz-Jeghers综合症，该疾病是一种常染色体显性遗传疾病，主要特征是良性胃肠息肉和口，足，手上的黑色皮肤损伤（6）。LKB1其它的一些突变跟几种组织中弥散的肿瘤有关（7）。

1. Baas, A.F. et al. (2004) Trends Cell Biol 14, 312-9.
2. Marignani, P.A. (2005) J Clin Pathol 58, 15-9.
3. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.
4. Hardie, D.G. (2004) J Cell Sci 117, 5479-87.
5. Sapkota, G.P. et al. (2002) Biochem J 362, 481-90.
6. Jenne, D.E. et al. (1998) Nat Genet 18, 38-43.
7. Sanchez-Cespedes, M. (2007) Oncogene 26, 7825-32.

原厂资料：

Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST''s product offering based upon superior performance in specified applications.

Background

LKB1 (STK11) is a serine/threonine kinase and tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regulation of numerous downstream kinases (1,2). A cytosolic protein complex comprised of LKB1, putative kinase STRAD, and the MO25 scaffold protein, activates both AMP-activated protein kinase (AMPK) and several AMPK-related kinases (3). AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations (4). LKB1 appears to be phosphorylated in cells at several sites, 
including human LKB1 at Ser31/325/428 and Thr189/336/363 (5). 
 Mutation in the corresponding LKB1 gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands and feet (6). A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues (7).

1. Baas, A.F. et al. (2004) Trends Cell Biol 14, 312-9.
2. Marignani, P.A. (2005) J Clin Pathol 58, 15-9.
3. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.
4. Hardie, D.G. (2004) J Cell Sci 117, 5479-87.
5. Sapkota, G.P. et al. (2002) Biochem J 362, 481-90.
6. Jenne, D.E. et al. (1998) Nat Genet 18, 38-43.
7. Sanchez-Cespedes, M. (2007) Oncogene 26, 7825-32.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody