FAM129B Antibody

• 产品编号：CST-5122S      品牌：CST       原厂货号：5122S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

FAM129B Antibody兔多抗能识别内源性总FAM129B蛋白。该多克隆抗体通过使用与人源Fam129B蛋白谷氨酸（705位点）附近残基相一致的合成肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。FAM129B/Niban-like protein 1（序列相似度 129的家族成员B）属于无显著特征的Niban蛋白家族，包括FAM129A/Niban、FAM129C/Niban-like protein 2。FAM129A/Niban蛋白参与ER应激反应，并且在甲状腺癌中蛋白水平被上调(1,2)。FAM129C/Niban-like protein 2在B细胞中优先表达，是五个生物标记物其中的一个，在大肠癌病人的全血中该蛋白水平被上调(3,4)。FAM129B蛋白广泛表达，被证实是黑色素瘤细胞中B-Raf蛋白的下游靶点(5)。尽管FAM129B蛋白不能调节细胞生长和分化，但是被B-Raf蛋白磷酸化的FAM129B蛋白对黑色素瘤细胞系和非黑色素瘤细胞系的侵袭潜能 十分重要(5)。黑色素瘤细胞中FAM129B蛋白的消除严重削弱了细胞外基质中B-Raf/MEK/Erk依赖型入侵(5)。

1. Sun, G.D. et al. (2007) Biochem Biophys Res Commun 360, 181-7.
2. Matsumoto, F. et al. (2006) Hum Pathol 37, 1592-600.
3. Boyd, R.S. et al. (2003) Leukemia 17, 1605-12.
4. Han, M. et al. (2008) Clin Cancer Res 14, 455-60.
5. Old, W.M. et al. (2009) Mol Cell 34, 115-31.

原厂资料：

Specificity / Sensitivity

FAM129B Antibody recognizes endogenous levels of total FAM129B protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu705 of human Fam129B protein. Antibodies were purified by protein A and peptide affinity chromatography.

Background

FAM129B/Niban-like protein 1 (family with sequence similarity 129, member B) belongs to a poorly characterized family of Niban proteins that also includes FAM129A/Niban and FAM129C/Niban-like protein 2. FAM129A/Niban is implicated in the ER stress response and is upregulated at the protein level in thyroid carcinoma (1,2). FAM129C/Niban-like protein 2 is preferentially expressed in B-cells and is one of five biomarkers upregulated in whole blood from patients with colorectal carcinoma (3,4). FAM129B is broadly expressed and has been shown to be a downstream target of B-Raf in melanoma cells (5). Though FAM129B does not appear to regulate cell growth and division, phosphorylation of FAM129B by B-Raf is essential for the invasive potential of melanoma and non-melanoma cell lines (5). Deletion of FAM129B in melanoma cells significantly impairs B-Raf/MEK/Erk-dependent invasion into the extracellular matrix (5).

1. Sun, G.D. et al. (2007) Biochem Biophys Res Commun 360, 181-7.
2. Matsumoto, F. et al. (2006) Hum Pathol 37, 1592-600.
3. Boyd, R.S. et al. (2003) Leukemia 17, 1605-12.
4. Han, M. et al. (2008) Clin Cancer Res 14, 455-60.
5. Old, W.M. et al. (2009) Mol Cell 34, 115-31.

注意事项：

Storage: Supplied in 10mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 μg/ml BSA and 50% glycerol. Store at -20ºC. Do not
aliquot the antibody