Argonaute 1 (D84G10) XP? Rabbit mAb

• 产品编号：CST-5053P      品牌：Cell Signaling Technology       原厂货号：5053P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Argonaute 1 (D84G10) XP® Rabbit mAb兔单抗检测内源性Argonaute 1总蛋白水平。通过人工合成人源Argonaute 1蛋白Ile392位点周围序列相应的多肽片段去免疫动物从而制备出单克隆抗体。在高等真核细胞中，小的非编码RNAs是重要的基因表达调节因子(1,2)。多种类别的小RNAs都已经被鉴定，例如短的干扰RNA(siRNAs)(3)，microRNAs (miRNAs)（4)和Piwi互作RNAs(piRNAs)(5)。MicroRNAs长度大约21核苷酸，其涉及到多种细胞内过程包括细胞生长、分化和应激反应(1,2)。MicroRNAs通过介导mRNA翻译或稳定来调节基因表达(2)。MicroRNAs与micro-ribonucleoproteins (miRNPs)复合物中的蛋白组分共同行使功能 (2)。在这些复合物中最重要一类重要成分就是Argonaute蛋白(1,2)。哺乳类Argonaute家族有四个成员，仅Argonaute 2(Ago2)拥有Slicer内切酶活性(1,2)。Argonaute蛋白在不同阶段参与microRNA介导基因沉默，例如翻译的抑制和mRNA降解(1)。

1. Peters, L. and Meister, G. (2007) Mol Cell 26, 611-23.
2. Filipowicz, W. et al. (2008) Nat Rev Genet 9, 102-14.
3. Caplen, N.J. et al. (2001) Proc Natl Acad Sci USA 98, 9742-7.
4. Mourelatos, Z. et al. (2002) Genes Dev 16, 720-8.
5. Girard, A. et al. (2006) Nature 442, 199-202.

原厂资料：

Specificity / Sensitivity

Argonaute 1 (D84G10) XP® Rabbit mAb detects endogenous levels of total Argonaute 1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence around Ile392 of human Argonaute 1 protein.

Background

Small non-coding RNAs are important regulators of gene expression in higher eukaryotes (1,2). Several classes of small RNAs, including short interfering RNAs (siRNAs) (3), microRNAs (miRNAs) (4), and Piwi-interacting RNAs (piRNAs) (5), have been identified. MicroRNAs are about 21 nucleotides in length and have been implicated in many cellular processes such as development, differentiation, and stress response (1,2). MicroRNAs regulate gene expression by modulating mRNA translation or stability (2). MicroRNAs function together with the protein components in the complexes called micro-ribonucleoproteins (miRNPs) (2). Among the most important components in these complexes are Argonaute proteins (1,2). There are four members in the mammalian Argonaute family and only Argonaute 2 (Ago2) possesses the Slicer endonuclease activity (1,2). Argonaute proteins participate in the various steps of microRNA-mediated gene silencing such as repression of translation and mRNA turnover (1).

1. Peters, L. and Meister, G. (2007) Mol Cell 26, 611-23.
2. Filipowicz, W. et al. (2008) Nat Rev Genet 9, 102-14.
3. Caplen, N.J. et al. (2001) Proc Natl Acad Sci USA 98, 9742-7.
4. Mourelatos, Z. et al. (2002) Genes Dev 16, 720-8.
5. Girard, A. et al. (2006) Nature 442, 199-202.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium
azide. Store at –20°C. Do not aliquot the antibody.