SGLT1 Antibody

• 产品编号：CST-5042S      品牌：CST       原厂货号：5042S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SGLT1 Antibody用于检测内源性的SGLT1总蛋白水平多克隆抗体是通过合成对应于人SGLT1蛋白Gly247位点序列的肽段免疫动物获得，抗体经Protein A和肽亲和层析纯化。钠离子/葡萄糖共转运蛋白（SGLT1）是一个葡萄糖主动转运蛋白，它利用钠离子的浓度梯度来将胞外的葡萄糖转运到胞内，而不依赖于胞外葡萄糖浓度。SGLT1是维持高的细胞内葡萄糖浓度的必需葡萄糖主动转运蛋白（1）。SGLT1主要在小肠和肾脏上皮表达，最近有研究发现在心肌中也发现了SGLT1的表达（2）。SGLT1的异常表达可能会导致2型糖尿病和心肌缺血（2）。SGLT1的一些突变会导致葡萄糖/半乳糖吸收障碍，这会引起新生儿腹泻，如果不治疗就会致死（3）。最近一些研究发现了EGFR在肿瘤细胞存活中的作用，EGFR会阻止自噬性细胞的死亡，而不依赖于EGFR的激酶活性，因为该受体与SLGT1结合，保证了细胞内葡萄糖的基本浓度（4）。

1. Wright, E.M. et al. (1994) J Exp Biol 196, 197-212.
2. Banerjee, S.K. et al. (2009) Cardiovasc Res 84, 111-8.
3. Martín, M.G. et al. (1996) Nat Genet 12, 216-20.
4. Weihua, Z. et al. (2008) Cancer Cell 13, 385-93.

原厂资料：

Specificity / Sensitivity

SGLT1 Antibody detects endogenous levels of total SGLT1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly247 of human SGLT1 protein. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Sodium/glucose cotransporter 1 (SGLT1) is an active glucose transporter, which utilizes sodium gradients to transport glucose into cells independent of extracellular glucose concentration. SGLT1 is an essential glucose active transport protein that helps maintain high intracellular glucose levels (1). Expression of SGLT1 is mainly seen in intestinal and kidney epithelial cells, although a recent study also characterized SGLT1 expression in cardiac myocytes (2). Abnormal SGLT1 expression may be associated with cases of type 2 diabetes mellitus and myocardial ischaemia (2). Mutation of the corresponding SGLT1 gene can result in congenital glucose/galactose malabsorption, which can lead to neonatal diarrhea and subsequent death if left untreated (3). A recent study of the role of EGFR in cancer cell survival indicates that EGFR can prevent autophagic cell death independent of EGFR kinase activity because the receptor interacts with and stabilizes SLGT1 to maintain basal intracellular glucose levels (4).

1. Wright, E.M. et al. (1994) J Exp Biol 196, 197-212.
2. Banerjee, S.K. et al. (2009) Cardiovasc Res 84, 111-8.
3. Martín, M.G. et al. (1996) Nat Genet 12, 216-20.
4. Weihua, Z. et al. (2008) Cancer Cell 13, 385-93.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.