Pan-Actin Antibody

• 产品编号：CST-4968S      品牌：CST       原厂货号：4968S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Pan-Actin抗体识别内源性的actin(所有亚型)总蛋白。该抗体也能识别glutamate 107位点剪切的30 kDa大小的actin片段。通过人工合成与人源beta-actin蛋白Asp244邻近氨基酸残基序列一致的肽段，免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Actin是一个广泛存在的真核细胞蛋白质，其实细胞骨架的重要组成成分。在哺乳类动物中至少有六种亚型。非肌肉组织β-和γ-actin也称作细胞质肌蛋白，它们在非肌肉组织中主要表达，目的是控制细胞结构和运动(1)。α-cardiac和α-skeletal 肌蛋白分别在心脏横纹肌和骨骼肌表达；两个平滑肌肌蛋白（α-和γ-actin）各自主要地发现在血管平滑肌和肠道平滑肌。这些肌蛋白亚型调节肌肉细胞的收缩潜能(1)。Actin主要存在作为一个纤维聚合物F-actin。在一些进程例如细胞分裂、内吞作用或应激中回应细胞骨架重组信号，cofilin促进分裂和F-actin的解聚，从而导致在单体球状形式G-actin的增加(2)。Arp2/3复合物使F-actin片段稳定，并且促进新的肌蛋白微丝形成(2)。有研究报道actin是在主要乳腺肿瘤中高度磷酸化(3)。在凋亡条件下肌蛋白的分裂已经在体外、心肌和骨骼肌中观察(4-6)。通过caspase-3剪切的Actin可能加速泛素/蛋白酶体依赖的肌肉蛋白水解(6)。

1. Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48-55.
2. Condeelis, J. (2001) Trends Cell Biol. 11, 288-293.
3. Lim, Y.P. et al. (2004) Clin. Cancer Res. 10, 3980-3987.
4. Kayalar, C. et al. (1996) Proc. Natl. Acad. Sci. USA. 93, 2234-2238.
5. Communal, C. et al. (2002) Proc. Natl. Acad. Sci. USA. 99, 6252-6256.
6. Du, J. et al. (2004) J. Clin. Invest. 113, 115-123.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: D. melanogaster, Xenopus, Bovine, Pig

Specificity / Sensitivity

Pan-Actin Antibody detects endogenous levels of total actin (all isoforms). The antibody also detects the 30 kDa actin fragment cleaved at glutamate 107.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp244 of human beta-actin. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle (4-6).

1. Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48-55.
2. Condeelis, J. (2001) Trends Cell Biol. 11, 288-293.
3. Lim, Y.P. et al. (2004) Clin. Cancer Res. 10, 3980-3987.
4. Kayalar, C. et al. (1996) Proc. Natl. Acad. Sci. USA. 93, 2234-2238.
5. Communal, C. et al. (2002) Proc. Natl. Acad. Sci. USA. 99, 6252-6256.
6. Du, J. et al. (2004) J. Clin. Invest. 113, 115-123.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.