Rap1A/Rap1B Antibody

• 产品编号：CST-4938S      品牌：CST       原厂货号：4938S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Rap1 Antibody检测内源性Rap1A和Rap1B总蛋白。该抗体不能与Rap2和其它Ras相关蛋白发生交叉反应。通过人工合成人源Rap1A蛋白Gln130位点相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Rap1和Rap2蛋白属于小GTPases的Ras亚家族，它们通过来自整合素、receptor tyrosine kinases (RTKs)、G-protein coupled receptors (GPCR)、death domain associated receptors (DD-R)和离子通道等多种刺激从而被激活(1,2)。像其它小GTPases一样，通过鸟嘌呤核苷酸交换因子(guanine nucleotide exchange factors,GEF)刺激Rap蛋白活性，并且通过GTPase activating proteins (GAP)失活。多种Rap GEFs已经被鉴定：通过受体蛋白例如Crk、来自cAMP的Epacs (or cAMP-GEFs)发出的信号和来自Ca2+和/或DAG的CD-GEFs (or CalDAG-GEFs)传递的信号，C3G与RTKs一起连接着Rap1蛋白(1)。Rap1主要调节多种整合素依赖的进程例如形态发生、细胞间的粘附、造血作用、白细胞迁移和肿瘤入侵 (1,2)。通过下游效应分子包括B-Raf、PI3K、RalGEF和phospholipases (PLCs)，Rap1蛋白也可能调节增殖、分化和生存 (1-4) 。Rap1和Rap2蛋白没有功能上冗余，因为它们进行重叠但明显的功能(5)。最近研究指出Rap2蛋白调节Dsh亚细胞定位，以及在早期发育中对Wnt信号是需要的(6)。

1. Bos, J. et al. (2001) Nat. Rev. Mol. Cell Biol. 2, 369-377.
2. Caron, E. (2003) J. Cell Sci. 116, 435-440.
3. Song, C. et al. (2002) Oncogene 21, 8105-8113.
4. Rong, R. et al. (2003) J. Biol. Chem. 278, 52497-52503.
5. Taira, K. et al. (2004) J. Biol. Chem. 279, 49488-49496.
6. Choi, S. and Han, J. (2005) EMBO J. 24, 985-996.

原厂资料：

Specificity / Sensitivity

Rap1 Antibody detects endogenous levels of total Rap1A and Rap1B protein. This antibody does not cross-react with Rap2 and other Ras-related proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln130 of human Rap1A. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Rap1 and Rap2 belong to the Ras subfamily of small GTPases and are activated by a wide variety of stimuli through integrins, receptor tyrosine kinases (RTKs), G-protein coupled receptors (GPCR), death domain associated receptors (DD-R) and ion channels (1,2). Like other small GTPases, Rap activity is stimulated by guanine nucleotide exchange factors (GEF) and inactivated by GTPase activating proteins (GAP). A wide variety of Rap GEFs have been identified: C3G connects Rap1 with RTKs through adaptor proteins such as Crk, Epacs (or cAMP-GEFs) transmit signals from cAMP, and CD-GEFs (or CalDAG-GEFs) convey signals from either or both Ca2+ and DAG (1). Rap1 primarily regulates multiple integrin-dependent processes such as morphogenesis, cell-cell adhesion, hematopoiesis, leukocyte migration and tumor invasion (1,2). Rap1 may also regulate proliferation, differentiation and survival through downstream effectors including B-Raf, PI3K, RalGEF and phospholipases (PLCs) (1-4). Rap1 and Rap2 are not fuctionally redundant as they perform overlapping but distinct functions (5). Recent research indicates that Rap2 regulates Dsh subcellular localization and is required for Wnt signaling in early development (6).

1. Bos, J. et al. (2001) Nat. Rev. Mol. Cell Biol. 2, 369-377.
2. Caron, E. (2003) J. Cell Sci. 116, 435-440.
3. Song, C. et al. (2002) Oncogene 21, 8105-8113.
4. Rong, R. et al. (2003) J. Biol. Chem. 278, 52497-52503.
5. Taira, K. et al. (2004) J. Biol. Chem. 279, 49488-49496.
6. Choi, S. and Han, J. (2005) EMBO J. 24, 985-996.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.