FAF1 Antibody

• 产品编号：CST-4932S      品牌：CST       原厂货号：4932S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

FAF1 Antibody能够检测内源性水平FAF1总蛋白。该多克隆抗体是采用合成的人源FAF1蛋白中心区域260位相对应的肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。FAF1是人们通过酵母双杂交筛选时首次发现的，其可与细胞质内的Fas发生相互作用，是TNF受体超家族的成员，在发育过程中的细胞凋亡和免疫功能中起着至关重要的作用（1）。研究观察到，FAF1广泛表达，在睾丸，骨骼肌和心脏中的表达量最高（2）。 FAF1会加强Fas介导的细胞凋亡，并且在某些类型细胞中能够在没有FAS刺激的情况下诱导细胞凋亡。它不具有典型的死亡基序，而是有两个与泛素结构同源的氨基酸末端结构域。虽然FAF1在细胞凋亡过程中的确切作用仍不清楚，但已确定它是Fas介导的细胞凋亡的死亡诱导信号复合物（DISC）的组分之一，并且可以结合到caspase-8和FADD上（3）。研究已经证明，FAF1能抑制NF-κB的转录因子激活（4）。

1. Chu, K. et al. (1995) Proc. Natl. Acad. Sci. 92, 11894-11898.
2. Ryu, S. W. et al. (1999) Biochem. Biophys. Res. Comm. 262, 388-394.
3. Ryu, S. W. et al. (2003) J. Biol. Chem. 278, 24003-24010.
4. Park, M. Y. et al. (2004) J. Biol. Chem. 279, 2544-2549.

原厂资料：

Specificity / Sensitivity

FAF1 Antibody detects endogenous levels of FAF1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to a central region of FAF1 around residue 260. Antibodies are purified by protein A and affinity chromatography.

Background

FAF1 was originally identified though yeast two-hybrid screening, interacting with the cytoplasmic domain of Fas, a member of the TNF receptor superfamily that plays a critical role in in apoptosis during development and immune function (1). FAF1 is widely expressed with highest expression observed in testis, skeletal muscle and heart (2). FAF1 potentiates Fas-mediated apoptosis and may induce apoptosis without Fas stimulation in some cell types. It does not contain typical death motifs, but rather has two amino-terminal domains with structural homology to ubiquitin. While the precise role of FAF1 during apoptosis is still unclear, it has been observed to be one of the components of the death-inducing signaling complex (DISC) during Fas-mediated apoptosis and can bind to caspase-8 and FADD (3). FAF1 has also been shown to suppress the activation of the NF-kappaB transcription factor (4).

1. Chu, K. et al. (1995) Proc. Natl. Acad. Sci. 92, 11894-11898.
2. Ryu, S. W. et al. (1999) Biochem. Biophys. Res. Comm. 262, 388-394.
3. Ryu, S. W. et al. (2003) J. Biol. Chem. 278, 24003-24010.
4. Park, M. Y. et al. (2004) J. Biol. Chem. 279, 2544-2549.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody