Ezh2 Antibody

• 产品编号：CST-4905S      品牌：CST       原厂货号：4905S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Ezh2 Antibody检能够测内源性Ezh2总蛋白水平。该抗体可能与Ezh2相关的蛋白发生交叉反应。通过合成的与人源Ezh2蛋白Arg354位点相应的多肽片段去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。polycomb group (PcG)蛋白是涉及维持数个发育调节基因的沉默水平，并且有助于维持细胞身份（cell identity）、细胞周期调节和肿瘤发生(1,2)。zeste homolog 2 (Ezh2)的增强子是一个该蛋白家族成员，它包含四种保守区域包括domain I、domain II、一个半胱氨酸富裕的氨基酸延伸序列和羧基端SET domain (3)。SET domain已经证明与histone methyltransferase (HMTase)活性有关联。此外，哺乳类Ezh2是一个组蛋白去乙酰酶复合物的成员之一，它在基因沉默中有功能，并且在染色质结构水平起着作用(4)。在体内，Ezh2复合物使histone H3在Lys9和27位点甲基化，这被认为是涉及到调节转录因子到特异的靶位点(5)。Ezh2蛋白在不同肿瘤形式中被下调，并且具有主要效应因子和肿瘤发生的调节因子的作用已经变成一个越来越感兴趣的课题(6)。

1. Sellers, W.R. and Loda, M. (2002) Cancer Cell 2, 349-50.
2. Visser, H.P. et al. (2001) Br J Haematol 112, 950-8.
3. Chen, H. et al. (1996) Genomics 38, 30-7.
4. Tonini, T. et al. (2004) Oncogene 23, 4930-7.
5. Müller, J. et al. (2002) Cell 111, 197-208.
6. Kleer, C.G. et al. (2003) Proc Natl Acad Sci U S A 100, 11606-11.

原厂资料：

Specificity / Sensitivity

Ezh2 Antibody detects endogenous levels of Ezh2 protein. The antibody may cross-react with Ezh2-related proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg354 of human Ezh2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The polycomb group (PcG) proteins are involved in maintaining the silenced state of several developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis (1,2). Enhancer of zeste homolog 2 (Ezh2), a member of this large protein family, contains four conserved regions including domain I, domain II, and a cysteine-rich amino acid stretch that precedes the carboxy-terminal SET domain (3). The SET domain has been linked with histone methyltransferase (HMTase) activity. Moreover, mammalian Ezh2 is a member of a histone deacetylase complex that functions in gene silencing, acting at the level of chromatin structure (4). Ezh2 complexes methylate histone H3 at Lys9 and 27 in vitro, which is thought to be involved in targeting transcriptional regulators to specific loci (5). Ezh2 is deregulated in various tumor types, and its role, both as a primary effector and as a mediator of tumorigenesis, has become a subject of increased interest (6).

1. Sellers, W.R. and Loda, M. (2002) Cancer Cell 2, 349-50.
2. Visser, H.P. et al. (2001) Br J Haematol 112, 950-8.
3. Chen, H. et al. (1996) Genomics 38, 30-7.
4. Tonini, T. et al. (2004) Oncogene 23, 4930-7.
5. Müller, J. et al. (2002) Cell 111, 197-208.
6. Kleer, C.G. et al. (2003) Proc Natl Acad Sci U S A 100, 11606-11.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.