Keratin 7 (R458) Antibody

• 产品编号：CST-4898S      品牌：CST       原厂货号：4898S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Keratin 7 (R458) 抗体识别内源性的keratin 7总蛋白。此单多克隆抗体由合成肽段免疫动物产生，该肽段与人keratin 7蛋白羧基末端的邻近氨基酸残基序列一致。通过蛋白A和多肽亲和层析纯化抗体。角蛋白（细胞角蛋白）是中间丝蛋白主要在上皮细胞中表达。角蛋白的异二聚体由一个酸性角蛋白（或type I角蛋白，角蛋白9-23或）和一个碱性角蛋白（type II角蛋白，角蛋白1-8）聚合形成中间丝（1.2）。角蛋白的亚型在组织-和分化的特异性表达，这使他们可做为有用的生物标志（1）。研究显示角蛋白基因突变与肌肤失调，肝脏和胰腺疾病，以及肠道炎性疾病相关（3-6）Keratin 7和Keratin 19蛋白存在于肝和胰腺的祖细胞/干细胞中。

1. Moll, R. et al. (1982) Cell 31, 11-24.
2. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
3. Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
4. Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
5. Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
6. Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.
7. Iyer, A. et al. (2008) Hum Pathol 39, 1370-7.
8. Meier, K. et al. (2009) Ann Anat 191, 70-82.

原厂资料：

Specificity / Sensitivity

Keratin 7 (R458) Antibody detects endogenous levels of total keratin 7 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human keratin 7 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).Keratin 7 and keratin 19 are present in hepatic and pancreatic progenitor/stem cells (7,8).

1. Moll, R. et al. (1982) Cell 31, 11-24.
2. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
3. Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
4. Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
5. Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
6. Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.
7. Iyer, A. et al. (2008) Hum Pathol 39, 1370-7.
8. Meier, K. et al. (2009) Ann Anat 191, 70-82.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody