PLK3 (D14F12) Rabbit mAb

• 产品编号：CST-4896P      品牌：Cell Signaling Technology       原厂货号：4896P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PLK3 (D14F12) Rabbit mAb 能够识别内源性PLK3总蛋白。该单克隆抗体是由合成的人源的针对PLK3蛋白半胱氨酸（625位）的肽段免疫动物生产的。 哺乳动物细胞中至少存在四种polo样激酶：PLK1，PLK2，PLK3和PLK4/SAK(1)。同其它的的PLK家族成员一样，PLK3含有一个氨基末端催化结构域和一个保守的羧基末端结构域称作Polo盒(2)。PLK3也被称为增殖相关激酶(Prk) (3)，最初被描述为成纤维生长因子(FGF)诱导型激酶(Fnk)而且被认定为响应FGF-1和其它有丝分裂原的即时早期反应基因(4)。PLK3是一种细胞因子诱导型丝氨酸/苏氨酸激酶，其蛋白表达受细胞周期调控。尽管发现其表达主要集中于细胞周期的G1期，细胞质分裂之前的分裂末期和G0期也可以检测到PLK3(5)。同其它的的PLK家族成员一样，PLK3主要发挥细胞周期调节子的功能。特别的是，S期的进入需要PLK3且它是G1期事件的关键调节子，RNAi诱导PLK3缺失的细胞体现了这一点(2)。PLK3也通过磷酸化p53第20位丝氨酸参与DNA损伤反应的调节(6)。在PI3K-缺陷小鼠的各种器官发生自发肿瘤时，PLK3可充当肿瘤抑制基因的角色(7)。和PLK1不同，PLK3在各种肿瘤如肺癌(3)、头颈部肿瘤(8)和肠癌(9)中的表达是下调的。

1. Nigg, E.A. (1998) Curr Opin Cell Biol 10, 776-83.
2. Zimmerman, W.C. and Erikson, R.L. (2007) Proc Natl Acad Sci USA 104, 1847-52.
3. Li, B. et al. (1996) J Biol Chem 271, 19402-8.
4. Donohue, P.J. et al. (1995) J Biol Chem 270, 10351-7.
5. Zimmerman, W.C. and Erikson, R.L. (2007) Cell Cycle 6, 1314-8.
6. Xie, S. et al. (2001) J Biol Chem 276, 43305-12.
7. Yang, Y. et al. (2008) Cancer Res 68, 4077-85.
8. Dai, W. et al. (2000) Genes Chromosomes Cancer 27, 332-6.
9. Dai, W. et al. (2002) Int J Oncol 20, 121-6.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat, Monkey

Specificity / Sensitivity

PLK3 (D14F12) Rabbit mAb detects endogenous levels of total PLK3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys625 of human PLK3 protein.

Background

At least 4 distinct polo-like kinases exist in mammalian cells: PLK1, PLK2, PLK3 and PLK4/SAK (1). Like the other PLK family members, PLK3 contains an amino-terminal catalytic domain and a conserved carboxy-terminal domain termed the Polo box (2). PLK3, also called proliferation-related kinase (Prk) (3), was originally described as a fibroblast growth factor (FGF)-inducible kinase (Fnk) and identified as an immediate-early response gene responsive to FGF-1 and other mitogens (4). PLK3 is a cytokine-inducible serine/threonine kinase whose protein expression is cell cycle regulated. Though its expression is found primarily in G1 phase of the cell cycle, PLK3 is detected in G0 and in late telophase prior to cytokinesis (5). Like the other PLK family members, PLK3 functions mainly as a regulator of the cell cycle. Specifically, PLK3 is required for entry into S phase and is a critical regulator of G1 events, as indicated by RNAi-induced PLK3-depleted cells (2). PLK3 is also involved in the regulation of DNA damage response via phosphorylation of p53 on Ser20 (6). PLK3 may act as a tumor suppressor as Plk3-deficient mice develop spontaneous tumors in various organs (7). Unlike PLK1, PLK3 expression is down regulated in cancers including lung (3), head and neck (8), and colon (9). 

1. Nigg, E.A. (1998) Curr Opin Cell Biol 10, 776-83.
2. Zimmerman, W.C. and Erikson, R.L. (2007) Proc Natl Acad Sci USA 104, 1847-52.
3. Li, B. et al. (1996) J Biol Chem 271, 19402-8.
4. Donohue, P.J. et al. (1995) J Biol Chem 270, 10351-7.
5. Zimmerman, W.C. and Erikson, R.L. (2007) Cell Cycle 6, 1314-8.
6. Xie, S. et al. (2001) J Biol Chem 276, 43305-12.
7. Yang, Y. et al. (2008) Cancer Res 68, 4077-85.
8. Dai, W. et al. (2000) Genes Chromosomes Cancer 27, 332-6.
9. Dai, W. et al. (2002) Int J Oncol 20, 121-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium
azide. Store at –20°C. Do not aliquot the antibody