Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor? 488 Conjugate)

• 产品编号：CST-4886S      品牌：CST       原厂货号：4886S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor®488 Conjugate)只能检测内源的在ser536位点磷酸化的NF-kappaB p65蛋白。此抗体不与p50亚单位或其他相关的蛋白交叉反应。此单克隆抗体是通过合成对应的NF-kappaB p65 Ser536位点周围的磷肽段来免疫动物而获得。此抗体在优化的条件下与Alexa Fluor® 488 偶联，F/P 比例为2-6。CST公司生产的此抗体与Alexa Fluor® 488荧光染料偶联，并通过人源细胞的流式细胞仪的测试。未偶联的抗体 #3033与人源，鼠源和大鼠的Phospho-NF-KappaB p65 (Ser536)蛋白反应，CST预测Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor® 488 Conjugate)也能识别这些物种的Phospho-NF-KappaB p65 (Ser536)蛋白。核因子κ B(NF-κB)/Rel 家族的转录调控因子在炎症反应和免疫反应中发挥了至关重要的作用(1,2)。在哺乳动物中一共有5个家族成员: RelA, c-Rel, RelB, NF-κB1 (p105/p50) 和 NF-κB2 (p100/p52)。 p105 和 p100 在蛋白水解酶的作用下分别形成p50 和 p52。Rel与p50和p52形成二聚体，此复合体能够结合到DNA上调控转录。在未刺激的状态下， NF-κB 在IκB抑制剂作用下在细胞质中处于非活性状态(3-5)。 NF-κB激活因子能够诱导 IκB 蛋白的磷酸化, 这就使IκB能够快速的经过泛素化-蛋白酶通路降解从而释放 NF-κB，激活的NF-κB入核调控基因的表达(6-8)。 NIK 和 IKKα (IKK1) 调节磷酸化并促使NF-κB2 (p100) 生成p52, p52之后会入核发挥功能(9-11)。

1. Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
2. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
3. Haskill, S. et al. (1991) Cell 65, 1281-9.
4. Thompson, J.E. et al. (1995) Cell 80, 573-82.
5. Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63.
8. Chen, Z.J. et al. (1996) Cell 84, 853-62.
9. Senftleben, U. et al. (2001) Science 293, 1495-9.
10. Coope, H.J. et al. (2002) EMBO J 21, 5375-85.
11. Xiao, G. et al. (2001) Mol Cell 7, 401-9.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Dog

Specificity / Sensitivity

Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor®488 Conjugate) detects NF-kappaB p65 only when phosphorylated at serine 536. It does not cross-react with the p50 subunit or other related proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser536 of human NF-kappaB p65. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.

Description

Cell Signaling Technology Antibody conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct Flow Cytometric analysis of human cells. The unconjugated antibody, #3033, reacts with Phospho-NF-KappaB p65 (Ser536) from human, mouse, and rat. CST expects that Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor® 488 Conjugate) will also recognize Phospho-NF-KappaB p65 (Ser536) in these species.

Background

Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).

1. Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
2. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
3. Haskill, S. et al. (1991) Cell 65, 1281-9.
4. Thompson, J.E. et al. (1995) Cell 80, 573-82.
5. Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63.
8. Chen, Z.J. et al. (1996) Cell 84, 853-62.
9. Senftleben, U. et al. (2001) Science 293, 1495-9.
10. Coope, H.J. et al. (2002) EMBO J 21, 5375-85.
11. Xiao, G. et al. (2001) Mol Cell 7, 401-9.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide, 2 mg/ml BSA. Store at 4°C. Protect from light.
Do not freeze.