FUS/TLS Antibody

• 产品编号：CST-4885S      品牌：CST       原厂货号：4885S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

FUS/TLS Antibody能够检测内源性的 FUS/TLS总蛋白水平。该多克隆抗体是经由合成的围绕人 TLS/FUS蛋白Gly272位点的氨基酸肽段免疫动物而生产的。经过Protein A和亲和层析纯化。FUS/TLS (fused in sarcoma/translocated in liposarcoma)最初是作为在许多癌症中发现的融合蛋白的一个组分而被鉴定出来的，包括黏液样脂肪肉瘤，急性髓系白血病以及Ewing’s肿瘤（1）。FUS/TLS能够与转录激活因子的DNA结合结构域融合，如CHOP和 ERG，致使那些研究者认为能导致肿瘤发生的目的基因异常转录（1-5）。FUS/TLS参与了一系列RNA加工事件，包括pre-mRNA剪接，mRNA转录和miRNA加工（1,6）。除了它在RNA代谢中的作用，FUS/TLS还能通过与不同转录因子的相互作用来维持基因组的稳定性以及共调控基因表达，比如核受体、YB-1、NF-κB 的p65亚基、TFIID以及 RUNX2 (1,6,7)。最近的研究发现在ALS (肌萎缩性脊髓侧索硬化) 和 FTLD (额颞叶退行性病变)病人中FUS/TLS有若干突变，导致 FUS/TLS在细胞质的错误定位(6,8-11)。

1. Yang, S. et al. (2010) Int J Biochem Cell Biol 42, 1408-11.
2. Crozat, A. et al. (1993) Nature 363, 640-4.
3. Rabbitts, T.H. et al. (1993) Nat Genet 4, 175-80.
4. Law, W.J. et al. (2006) Brief Funct Genomic Proteomic 5, 8-14.
5. Prasad, D.D. et al. (1994) Oncogene 9, 3717-29.
6. Lagier-Tourenne, C. et al. (2010) Hum Mol Genet 19, R46-64.
7. Baechtold, H. et al. (1999) J Biol Chem 274, 34337-42.
8. Hewitt, C. et al. (2010) Arch Neurol 67, 455-61.
9. Vance, C. et al. (2009) Science 323, 1208-11.
10. Van Langenhove, T. et al. (2010) Neurology 74, 366-71.
11. Da Cruz, S. and Cleveland, D.W. (2011) Curr Opin Neurobiol 21, 904-19.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Hamster, Bovine, Guinea Pig, Horse

Specificity / Sensitivity

FUS/TLS Antibody recognizes endogenous levels of total FUS/TLS protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly272 of human TLS/FUS protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

FUS/TLS (fused in sarcoma/translocated in liposarcoma) was initially identified by investigators as a component of fusion proteins found in a variety of cancers such as myxoid liposarcoma, acute myeloid leukemia, and Ewing’s tumor (1). FUS/TLS fusion with the DNA binding domain of transcription activators such as CHOP and ERG leads to aberrant transcription of target genes that is thought by researchers to lead to tumor development (1-5). FUS/TLS is involved in a wide range of RNA processing events such as pre-mRNA splicing, mRNA transcription, and miRNA processing (1,6). In addition to its role in RNA metabolism, FUS/TLS maintains genomic stability and co-regulates gene expression by interacting with various transcription factors such as nuclear receptors, YB-1, p65 subunit of NF-κB, TFIID, and RUNX2 (1,6,7). More recently, researchers have found several mutations of FUS/TLS in ALS (amyotrophic lateral sclerosis) and FTLD (frontotemporal lobar degeneration) patients that causes cytoplasmic mislocalization of FUS/TLS (6,8-11).

1. Yang, S. et al. (2010) Int J Biochem Cell Biol 42, 1408-11.
2. Crozat, A. et al. (1993) Nature 363, 640-4.
3. Rabbitts, T.H. et al. (1993) Nat Genet 4, 175-80.
4. Law, W.J. et al. (2006) Brief Funct Genomic Proteomic 5, 8-14.
5. Prasad, D.D. et al. (1994) Oncogene 9, 3717-29.
6. Lagier-Tourenne, C. et al. (2010) Hum Mol Genet 19, R46-64.
7. Baechtold, H. et al. (1999) J Biol Chem 274, 34337-42.
8. Hewitt, C. et al. (2010) Arch Neurol 67, 455-61.
9. Vance, C. et al. (2009) Science 323, 1208-11.
10. Van Langenhove, T. et al. (2010) Neurology 74, 366-71.
11. Da Cruz, S. and Cleveland, D.W. (2011) Curr Opin Neurobiol 21, 904-19.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.