HSP40 (C64B4) Rabbit mAb

• 产品编号：CST-4871P      品牌：Cell Signaling Technology       原厂货号：4871P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

HSP40 (C64B4) Rabbit mAb兔单抗识别内源性的HSP40总蛋白。此抗体与其它HSP蛋白没有交叉反应。该单克隆抗体用与人类HSP40/Hdj1蛋白Glu223位点附近氨基酸序列对应的人工合成肽段免疫动物而制成。HSP40和HSP40类似的蛋白代表了很大一类分子伴侣家族蛋白，它们与大肠杆菌DnaJ蛋白同源（1）。这些蛋白根据其结构分为不同亚型。这个家族通常具有一个保守的J结构域，它通常定位于蛋白的氨基末端，负责与HSP70（1，2）结合。人HSP40也称为Hdj1，属于II型，包含一个独特的甘氨酸/苯丙氨酸富集区域（2）。HSP40家族的蛋白质可以与未折叠蛋白质结合，防止其聚集，并将未折叠蛋白质输送至HSP70（2，3）。HSP40的另一个主要功能是激活HSP70的ATP酶活性，导致未结合蛋白质的构象改变（4，5）。HSP40-HSP70未折叠蛋白复合物进一步结合共分子伴侣Hip、Hop和HSP90或者蛋白降解装置的组分如CHIP和BAG-1，分别导致蛋白折叠或降解（6）。

1. Cheetham, M.E. and Caplan, A.J. (1998) Cell Stress Chaperones 3, 28-36.
2. Fan, C.Y. et al. (2003) Cell Stress Chaperones 8, 309-316.
3. Langer, T. et al. (1992) Nature 356, 683-689.
4. Liberek, K. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 2874-2878.
5. Cyr, D.M. et al. (1992) J. Biol. Chem. 267, 20927-20931.
6. Höhfeld, J. et al. (2001) EMBO Rep. 2, 885-890.

原厂资料：

Specificity / Sensitivity

HSP40 (C64B4) Rabbit mAb detects endogenous levels of total HSP40 protein. This antibody does not cross-react with other HSPs.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to Glu223 of human HSP40/Hdj1.

Background

HSP40 and HSP40-like proteins represent a large family of chaperone proteins that are homologous to E. coli DnaJ protein (1). These proteins are classified into three subtypes based on their structures. The common feature of the family is a conserved J domain, which is usually located at the amino terminus of proteins and responsible for their association with HSP70 (1,2). Human HSP40, also known as Hdj1, belongs to subtype II that contain a unique Gly/Phe-rich region (2). HSP40 family proteins bind unfolded proteins, prevent their aggregation, and then deliver them to HSP70 (2,3). Another major function of HSP40 is to stimulate ATPase activity of HSP70, which causes conformational change of the unfolded proteins (4,5). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 or components of the protein degradation machinery such as CHIP and BAG-1, which either leads to protein folding or degradation, respectively (6).

1. Cheetham, M.E. and Caplan, A.J. (1998) Cell Stress Chaperones 3, 28-36.
2. Fan, C.Y. et al. (2003) Cell Stress Chaperones 8, 309-316.
3. Langer, T. et al. (1992) Nature 356, 683-689.
4. Liberek, K. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 2874-2878.
5. Cyr, D.M. et al. (1992) J. Biol. Chem. 267, 20927-20931.
6. Höhfeld, J. et al. (2001) EMBO Rep. 2, 885-890.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.